Adenovirus-dependent release of choline from plasma membrane vesicles at an acidic pH is mediated by the penton base protein - PubMed (original) (raw)
Adenovirus-dependent release of choline from plasma membrane vesicles at an acidic pH is mediated by the penton base protein
P Seth. J Virol. 1994 Feb.
Abstract
It has been suggested that during receptor-mediated endocytosis of human adenovirus (Ad) type 2 into cells, Ad disrupts the membrane of endocytic vesicles to enter the cytosol. To understand the mechanism of Ad-mediated disruption of the endocytic vesicles, I exposed Ad to plasma membrane vesicles derived from KB cells. Ad caused release of choline from the plasma membrane vesicles preloaded with [3H]choline. The efflux of choline was dependent on (i) the concentration of Ad, with a half-maximal effect at 0.5 microgram/ml; (ii) the pH of the buffer, with the optimum pH of the reaction ranging from 5.5 to 6.0; (iii) the length of the incubation, with a half-maximal release at 2 min; and (iv) the temperature of the incubation, with the optimum temperature being 37 degrees C. The Ad-dependent release of choline was inhibited by anti-penton base, while antihexon did not block the effect. These results suggest roles for a low-pH environment and the penton base protein in the Ad-dependent efflux of choline from plasma membrane vesicles.
Similar articles
- Active and separate secretion of fiber and penton base during the early phase of Ad2 or Ad5 infection.
Yan Y, Zhang B, Hou W, Lin H, Rebetz J, Hong SS, Wang Y, Ran L, Fan X. Yan Y, et al. Virology. 2017 May;505:172-180. doi: 10.1016/j.virol.2017.02.018. Epub 2017 Mar 4. Virology. 2017. PMID: 28264780 - Binding of adenovirus and its external proteins to Triton X-114. Dependence on pH.
Seth P, Willingham MC, Pastan I. Seth P, et al. J Biol Chem. 1985 Nov 25;260(27):14431-4. J Biol Chem. 1985. PMID: 4055783 - Role of vesicles during adenovirus 2 internalization into HeLa cells.
Svensson U. Svensson U. J Virol. 1985 Aug;55(2):442-9. doi: 10.1128/JVI.55.2.442-449.1985. J Virol. 1985. PMID: 2862291 Free PMC article. - Novel partner proteins of adenovirus penton.
Chroboczek J, Gout E, Favier AL, Galinier R. Chroboczek J, et al. Curr Top Microbiol Immunol. 2003;272:37-55. doi: 10.1007/978-3-662-05597-7_2. Curr Top Microbiol Immunol. 2003. PMID: 12747546 Review. - Endocytosis of adenovirus and adenovirus capsid proteins.
Medina-Kauwe LK. Medina-Kauwe LK. Adv Drug Deliv Rev. 2003 Nov 14;55(11):1485-96. doi: 10.1016/j.addr.2003.07.010. Adv Drug Deliv Rev. 2003. PMID: 14597142 Review.
Cited by
- Influence of cell physiological state on gene delivery to T lymphocytes by chimeric adenovirus Ad5F35.
Zhang WF, Shao HW, Wu FL, Xie X, Li ZM, Bo HB, Shen H, Wang T, Huang SL. Zhang WF, et al. Sci Rep. 2016 Mar 14;6:22688. doi: 10.1038/srep22688. Sci Rep. 2016. PMID: 26972139 Free PMC article. - Adenovirus membrane penetration: Tickling the tail of a sleeping dragon.
Wiethoff CM, Nemerow GR. Wiethoff CM, et al. Virology. 2015 May;479-480:591-9. doi: 10.1016/j.virol.2015.03.006. Epub 2015 Mar 19. Virology. 2015. PMID: 25798531 Free PMC article. Review. - Coat as a dagger: the use of capsid proteins to perforate membranes during non-enveloped DNA viruses trafficking.
Bilkova E, Forstova J, Abrahamyan L. Bilkova E, et al. Viruses. 2014 Jul 23;6(7):2899-937. doi: 10.3390/v6072899. Viruses. 2014. PMID: 25055856 Free PMC article. Review. - Development of adenovirus capsid proteins for targeted therapeutic delivery.
Medina-Kauwe LK. Medina-Kauwe LK. Ther Deliv. 2013 Feb;4(2):267-77. doi: 10.4155/tde.12.155. Ther Deliv. 2013. PMID: 23343164 Free PMC article. Review. - Functional genetic and biophysical analyses of membrane disruption by human adenovirus.
Moyer CL, Wiethoff CM, Maier O, Smith JG, Nemerow GR. Moyer CL, et al. J Virol. 2011 Mar;85(6):2631-41. doi: 10.1128/JVI.02321-10. Epub 2011 Jan 5. J Virol. 2011. PMID: 21209115 Free PMC article.
References
- Mol Cell Biol. 1984 Aug;4(8):1528-33 - PubMed
- J Virol. 1984 Sep;51(3):650-5 - PubMed
- J Biol Chem. 1985 Aug 15;260(17):9598-602 - PubMed
- J Virol. 1985 Aug;55(2):442-9 - PubMed
- J Biol Chem. 1985 Nov 25;260(27):14431-4 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources