Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae - PubMed (original) (raw)

Protein kinase C activators inhibit receptor-mediated potocytosis by preventing internalization of caveolae

E J Smart et al. J Cell Biol. 1994 Feb.

Abstract

Potocytosis is an endocytic pathway that utilizes glycosylphosphatidylinositol-anchored membrane proteins and caveolae to concentrate and internalize small molecules. We now report that activators of protein kinase C are potent inhibitors of potocytosis. Activators such as phorbol-12-myristate-13-acetate (PMA) inhibit the internalization of receptors for 5-methyltetrahydrofolate but allow the internal receptor pool to return to the cell surface. PMA does not affect the clustering of the folate receptor but instead markedly reduces the number of caveolae. Exposure to PMA totally blocks the intracellular accumulation of 5-methyltetrahydrofolate without affecting receptor-independent uptake or the formation of polyglutamylated species of 5-methyltetrahydrofolate in the cytoplasm. These data suggest that PMA inhibits uptake by inactivating caveolae internalization.

PubMed Disclaimer

Similar articles

• PMA alters folate receptor distribution in the plasma membrane and increases the rate of 5-methyltetrahydrofolate delivery in mature MA104 cells.
  Lewis CM, Smith AK, Nguyen C, Kamen BA. Lewis CM, et al. Biochim Biophys Acta. 1998 Feb 4;1401(2):157-69. doi: 10.1016/s0167-4889(97)00126-2. Biochim Biophys Acta. 1998. PMID: 9531971
• Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells.
  Smart EJ, Mineo C, Anderson RG. Smart EJ, et al. J Cell Biol. 1996 Sep;134(5):1169-77. doi: 10.1083/jcb.134.5.1169. J Cell Biol. 1996. PMID: 8794859 Free PMC article.
• Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.
  Chang WJ, Rothberg KG, Kamen BA, Anderson RG. Chang WJ, et al. J Cell Biol. 1992 Jul;118(1):63-9. doi: 10.1083/jcb.118.1.63. J Cell Biol. 1992. PMID: 1618907 Free PMC article.
• Potocytosis: sequestration and transport of small molecules by caveolae.
  Anderson RG, Kamen BA, Rothberg KG, Lacey SW. Anderson RG, et al. Science. 1992 Jan 24;255(5043):410-1. doi: 10.1126/science.1310359. Science. 1992. PMID: 1310359 Review. No abstract available.
• A review of folate receptor alpha cycling and 5-methyltetrahydrofolate accumulation with an emphasis on cell models in vitro.
  Kamen BA, Smith AK. Kamen BA, et al. Adv Drug Deliv Rev. 2004 Apr 29;56(8):1085-97. doi: 10.1016/j.addr.2004.01.002. Adv Drug Deliv Rev. 2004. PMID: 15094208 Review.

Cited by

• The folate receptor as a potential therapeutic anticancer target.
  Gruner BA, Weitman SD. Gruner BA, et al. Invest New Drugs. 1998-1999;16(3):205-19. doi: 10.1023/a:1006147932159. Invest New Drugs. 1998. PMID: 10360600 Review. No abstract available.
• Endocytosis of GPI-anchored proteins in human lymphocytes: role of glycolipid-based domains, actin cytoskeleton, and protein kinases.
  Deckert M, Ticchioni M, Bernard A. Deckert M, et al. J Cell Biol. 1996 May;133(4):791-9. doi: 10.1083/jcb.133.4.791. J Cell Biol. 1996. PMID: 8666664 Free PMC article.
• A detergent-free method for purifying caveolae membrane from tissue culture cells.
  Smart EJ, Ying YS, Mineo C, Anderson RG. Smart EJ, et al. Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10104-8. doi: 10.1073/pnas.92.22.10104. Proc Natl Acad Sci U S A. 1995. PMID: 7479734 Free PMC article.
• Caveolin-1, a master regulator of cellular senescence.
  Volonte D, Galbiati F. Volonte D, et al. Cancer Metastasis Rev. 2020 Jun;39(2):397-414. doi: 10.1007/s10555-020-09875-w. Cancer Metastasis Rev. 2020. PMID: 32279119 Free PMC article. Review.
• Opioid-Modulated Receptor Localization and Erk1/2 Phosphorylation in Cells Coexpressing μ-Opioid and Nociceptin Receptors.
  Zhuo GY, Chen MC, Lin TY, Lin ST, Chen DT, Lee CW. Zhuo GY, et al. Int J Mol Sci. 2023 Jan 5;24(2):1048. doi: 10.3390/ijms24021048. Int J Mol Sci. 2023. PMID: 36674576 Free PMC article.

References

1. 1. J Cell Biol. 1992 Dec;119(6):1507-13 - PubMed
2. 1. Cell. 1983 May;33(1):273-85 - PubMed
3. 1. Prog Lipid Res. 1990;29(3):141-66 - PubMed
4. 1. J Cell Biol. 1989 Jun;108(6):2401-8 - PubMed
5. 1. Biochem Biophys Res Commun. 1987 Dec 31;149(3):1084-92 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information