New monoclonal antibodies directed against mouse follicular dendritic cells - PubMed (original) (raw)
New monoclonal antibodies directed against mouse follicular dendritic cells
N Imazeki et al. J Histochem Cytochem. 1994 Mar.
Abstract
The follicular dendritic cells (FDC) in B-lymphoid follicles are unique reticular cells that retain immune complexes on their surfaces. We developed new monoclonal antibodies (MAb), SKY01, 28, 41, and 49, against mouse FDC without useful cell markers. Immunohistochemical study of spleen and lymph node tissues from Balb/c, C3H, and C57BL/6 mice revealed that SKY01 and 49 were highly specific for FDC, whereas SKY28 and 41 reacted with other stromal components as well as FDC. On immunoelectron microscopy (IEM), reaction products for all MAb were localized on the surfaces of FDC. In ontogenetic study of Balb/c spleen, FDC precursors were not immunodetected with any of the MAb at 1 week after birth. The FDC that first appeared as immune complex-retaining cells at 2 weeks were a subpopulation of SKY01-, 49-, 28+, and 41+ reticular cells. At 3 weeks, FDC were positive for all MAb, like adult spleen. These results indicate that our MAb recognize differentiation antigens of FDC. Comparative immunohistochemical studies of spleen from athymic nude and severe combined immunodeficiency mice suggested that B-cells may be required for the differentiation of FDC. Consequently, these MAb are considered useful tools for research on FDC.
Similar articles
- Host origin of follicular dendritic cells induced in the spleen of SCID mice after transfer of allogeneic lymphocytes.
Yoshida K, Kaji M, Takahashi T, van den Berg TK, Dijkstra CD. Yoshida K, et al. Immunology. 1995 Jan;84(1):117-26. Immunology. 1995. PMID: 7890295 Free PMC article. - Ontogeny of the follicular dendritic cell phenotype and function in the postnatal murine spleen.
Balogh P, Aydar Y, Tew JG, Szakal AK. Balogh P, et al. Cell Immunol. 2001 Nov 25;214(1):45-53. doi: 10.1006/cimm.2001.1874. Cell Immunol. 2001. PMID: 11902828 - Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII).
Maeda K, Burton GF, Padgett DA, Conrad DH, Huff TF, Masuda A, Szakal AK, Tew JG. Maeda K, et al. J Immunol. 1992 Apr 15;148(8):2340-7. J Immunol. 1992. PMID: 1532811 - [Follicular dendritic cells: phenotype, origin and functions].
Heinen E. Heinen E. Pathol Biol (Paris). 1995 Dec;43(10):848-57. Pathol Biol (Paris). 1995. PMID: 8786889 Review. French. - Characterizing follicular dendritic cells: A progress report.
Aguzzi A, Krautler NJ. Aguzzi A, et al. Eur J Immunol. 2010 Aug;40(8):2134-8. doi: 10.1002/eji.201040765. Eur J Immunol. 2010. PMID: 20853499 Review.
Cited by
- Development, maturation and subsequent activation of follicular dendritic cells (FDC): immunohistochemical observation of human fetal and adult lymph nodes.
Kasajima-Akatsuka N, Maeda K. Kasajima-Akatsuka N, et al. Histochem Cell Biol. 2006 Aug;126(2):261-73. doi: 10.1007/s00418-006-0157-6. Epub 2006 Feb 10. Histochem Cell Biol. 2006. PMID: 16470387 - Involvement of myeloid dendritic cells in the development of gastric secondary lymphoid follicles in Helicobacter pylori-infected neonatally thymectomized BALB/c mice.
Nishi T, Okazaki K, Kawasaki K, Fukui T, Tamaki H, Matsuura M, Asada M, Watanabe T, Uchida K, Watanabe N, Nakase H, Ohana M, Hiai H, Chiba T. Nishi T, et al. Infect Immun. 2003 Apr;71(4):2153-62. doi: 10.1128/IAI.71.4.2153-2162.2003. Infect Immun. 2003. PMID: 12654837 Free PMC article. - The sequential role of lymphotoxin and B cells in the development of splenic follicles.
Gonzalez M, Mackay F, Browning JL, Kosco-Vilbois MH, Noelle RJ. Gonzalez M, et al. J Exp Med. 1998 Apr 6;187(7):997-1007. doi: 10.1084/jem.187.7.997. J Exp Med. 1998. PMID: 9529316 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources