Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces - PubMed (original) (raw)

Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces

H F Jenkinson et al. Infect Immun. 1993 Aug.

Abstract

Cell surface protein SSP-5 in the oral bacterium Streptococcus gordonii M5 binds human salivary agglutinin in a Ca(2+)-dependent reaction (D.R. Demuth, E.E. Golub, and D. Malamud, J. Biol. Chem. 265:7120-7126, 1990). The region of the gene encoding an N-terminal segment of a related polypeptide (SspA) in S. gordonii DL1 (Challis) was isolated following polymerase chain reaction amplification of genomic DNA. The sspA gene in S. gordonii DL1 was insertionally inactivated by homologous recombination of the erythromycin resistance (Emr) determinant ermAM onto the streptococcal chromosome. The SspA polypeptide (apparent molecular mass, 210 kDa) was detected on Western blots (immunoblots) of spheroplast extracts and extracellular culture medium proteins from wild-type strain DL1 but was absent from Emr mutants. One SspA- mutant (designated OB220) was not altered in rate or extent of aggregation by whole saliva or parotid saliva but showed reduced aggregation in the presence of purified salivary agglutinin. Mutant bacteria were unaffected in their ability to adhere to hydroxylapatite beads coated with whole or parotid saliva and were unaltered in cell surface hydrophobicity. However, the SspA- strain OB220 was deficient in binding salivary agglutinin and in binding to six strains of Actinomyces naeslundii. Therefore, expression of SspA polypeptide in S. gordonii is associated with both agglutinin-dependent and agglutinin-independent aggregation and adherence reactions of streptococcal cells.

PubMed Disclaimer

Cited by

Streptococcus adherence and colonization.
Nobbs AH, Lamont RJ, Jenkinson HF. Nobbs AH, et al. Microbiol Mol Biol Rev. 2009 Sep;73(3):407-50, Table of Contents. doi: 10.1128/MMBR.00014-09. Microbiol Mol Biol Rev. 2009. PMID: 19721085 Free PMC article. Review.
Adherence of Candida albicans to a cell surface polysaccharide receptor on Streptococcus gordonii.
Holmes AR, Gopal PK, Jenkinson HF. Holmes AR, et al. Infect Immun. 1995 May;63(5):1827-34. doi: 10.1128/iai.63.5.1827-1834.1995. Infect Immun. 1995. PMID: 7729891 Free PMC article.
Effects of Nicotine on Streptococcus gordonii Growth, Biofilm Formation, and Cell Aggregation.
Huang R, Li M, Ye M, Yang K, Xu X, Gregory RL. Huang R, et al. Appl Environ Microbiol. 2014 Dec;80(23):7212-8. doi: 10.1128/AEM.02395-14. Epub 2014 Sep 12. Appl Environ Microbiol. 2014. PMID: 25217021 Free PMC article.
Identification of salivary mucin MUC7 binding proteins from Streptococcus gordonii.
Kesimer M, Kiliç N, Mehrotra R, Thornton DJ, Sheehan JK. Kesimer M, et al. BMC Microbiol. 2009 Aug 11;9:163. doi: 10.1186/1471-2180-9-163. BMC Microbiol. 2009. PMID: 19671172 Free PMC article.
The effect of smoking on caries-related microorganisms.
Wu J, Li M, Huang R. Wu J, et al. Tob Induc Dis. 2019 Apr 18;17:32. doi: 10.18332/tid/105913. eCollection 2019. Tob Induc Dis. 2019. PMID: 31516475 Free PMC article. Review.

References

1. Oral Microbiol Immunol. 1988 Dec;3(4):162-8 - PubMed
1. J Dent Res. 1962 Jul-Aug;41:778-83 - PubMed
1. Oral Microbiol Immunol. 1989 Jun;4(2):106-11 - PubMed
1. Infect Immun. 1989 Nov;57(11):3306-13 - PubMed
1. FEBS Lett. 1989 Nov 20;258(1):127-32 - PubMed

Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces - PubMed (original) (raw)