Specific commitment of different pre-mRNAs to splicing by single SR proteins - PubMed (original) (raw)
. 1993 Sep 2;365(6441):82-5.
doi: 10.1038/365082a0.
Affiliations
- PMID: 8361546
- DOI: 10.1038/365082a0
Specific commitment of different pre-mRNAs to splicing by single SR proteins
X D Fu. Nature. 1993.
Abstract
Higher eukaryotic cells express a family of essential splicing factors with a characteristic RNA-binding domain and serine/arginine-rich (SR) motif. These SR proteins, which include SC35 and SF2/ASF, are conserved from Drosophila to man, are required for early steps of spliceosome assembly and can influence splice-site selections. To address their mechanisms of action, SR proteins were examined for their role in committing pre-messenger RNA to the splicing pathway. I report here that SC35 was sufficient on its own to form a committed complex with human beta-globin pre-mRNA. Examination of other SR proteins and pre-mRNA substrates revealed that single SR proteins committed different pre-mRNAs to splicing with pronounced substrate specificity. These results suggest that splicing of different pre-mRNAs may require distinct sets of SR proteins, and that the commitment by SR proteins may be a critical step at which alternative and tissue-specific splicing is regulated.
Comment in
- RNA splicing. Question of commitment.
Manley J. Manley J. Nature. 1993 Sep 2;365(6441):14. doi: 10.1038/365014a0. Nature. 1993. PMID: 8361529 No abstract available.
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