Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain - PubMed (original) (raw)
Affiliations
- PMID: 8361765
Comparative Study
Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain
X Mayol et al. Oncogene. 1993 Sep.
Abstract
The product of the retinoblastoma tumor suppressor gene (pRb) and p107 share a high degree of structural homology in the pocket region, which is thought to play a primary role in the function of these proteins. It is conceivable that there exists a larger family of cellular proteins containing this pocket region. In this communication, we report cloning of a new human cDNA encoding a polypeptide that shows a high level of identity with pRb and p107 and possesses a pocket region. We have named it pRb2. From the deduced amino acid sequence, pRb2 has a predicted molecular weight of approximately 120 kD and its in vitro translated product binds to the adenovirus E1A protein. Due to its size, pRb2 may correspond to p130, which has previously been shown by us to interact with the transforming region of E1A in in vivo studies. Interestingly, pRb2 fails to bind an E1A mutant in the transforming domain 2 suggesting that pRb2 is involved in the transforming capacity of E1A.
Similar articles
- Viral oncoproteins E1A and E7 and cellular LxCxE proteins repress SUMO modification of the retinoblastoma tumor suppressor.
Ledl A, Schmidt D, Müller S. Ledl A, et al. Oncogene. 2005 May 26;24(23):3810-8. doi: 10.1038/sj.onc.1208539. Oncogene. 2005. PMID: 15806172 - The retinoblastoma gene family members pRB and p107 coactivate the AP-1-dependent mouse tissue factor promoter in fibroblasts.
Liu SL, Rand A, Kelm RJ Jr, Getz MJ. Liu SL, et al. Oncogene. 2000 Jul 13;19(30):3352-62. doi: 10.1038/sj.onc.1203675. Oncogene. 2000. PMID: 10918592 - Conserved region 2 of adenovirus E1A has a function distinct from pRb binding required to prevent cell cycle arrest by p16INK4a or p27Kip1.
Alevizopoulos K, Sanchez B, Amati B. Alevizopoulos K, et al. Oncogene. 2000 Apr 13;19(16):2067-74. doi: 10.1038/sj.onc.1203534. Oncogene. 2000. PMID: 10803468 - Tumor suppressor pRb2/p130 gene and its derived product Spa310 spacer domain as perspective candidates for cancer therapy.
Giordano A, Rossi A, Romano G, Bagella L. Giordano A, et al. J Cell Physiol. 2007 Nov;213(2):403-6. doi: 10.1002/jcp.21225. J Cell Physiol. 2007. PMID: 17708530 Review. - Retinoblastoma family proteins as key targets of the small DNA virus oncoproteins.
Felsani A, Mileo AM, Paggi MG. Felsani A, et al. Oncogene. 2006 Aug 28;25(38):5277-85. doi: 10.1038/sj.onc.1209621. Oncogene. 2006. PMID: 16936748 Review.
Cited by
- Aurora kinase A revives dormant laryngeal squamous cell carcinoma cells via FAK/PI3K/Akt pathway activation.
Yang LY, He CY, Chen XH, Su LP, Liu BY, Zhang H. Yang LY, et al. Oncotarget. 2016 Jul 26;7(30):48346-48359. doi: 10.18632/oncotarget.10233. Oncotarget. 2016. PMID: 27356739 Free PMC article. - Skin Tumors Rb(eing) Uncovered.
Costa C, Paramio JM, Santos M. Costa C, et al. Front Oncol. 2013 Dec 17;3:307. doi: 10.3389/fonc.2013.00307. Front Oncol. 2013. PMID: 24381932 Free PMC article. Review. - Loss of the retinoblastoma protein-related p130 protein in small cell lung carcinoma.
Helin K, Holm K, Niebuhr A, Eiberg H, Tommerup N, Hougaard S, Poulsen HS, Spang-Thomsen M, Norgaard P. Helin K, et al. Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6933-8. doi: 10.1073/pnas.94.13.6933. Proc Natl Acad Sci U S A. 1997. PMID: 9192669 Free PMC article. - Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism.
Chestukhin A, Litovchick L, Rudich K, DeCaprio JA. Chestukhin A, et al. Mol Cell Biol. 2002 Jan;22(2):453-68. doi: 10.1128/MCB.22.2.453-468.2002. Mol Cell Biol. 2002. PMID: 11756542 Free PMC article. - Domains A and B in the Rb pocket interact to form a transcriptional repressor motif.
Chow KN, Dean DC. Chow KN, et al. Mol Cell Biol. 1996 Sep;16(9):4862-8. doi: 10.1128/MCB.16.9.4862. Mol Cell Biol. 1996. PMID: 8756645 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Molecular Biology Databases