Morphological change and destabilization of beta-actin mRNA by tumor necrosis factor in human microvascular endothelial cells - PubMed (original) (raw)
. 1993 Oct;208(2):498-503.
doi: 10.1006/excr.1993.1272.
Affiliations
- PMID: 8375478
- DOI: 10.1006/excr.1993.1272
Morphological change and destabilization of beta-actin mRNA by tumor necrosis factor in human microvascular endothelial cells
K Kohno et al. Exp Cell Res. 1993 Oct.
Abstract
Tumor necrosis factor-alpha (TNF-alpha) produced morphological changes from a cobblestone-like shape into a spindle shape in human omental microvascular endothelial (HOME) cells and also a drastic rearrangement of actin filaments. Expression of beta-actin gene was diminished in HOME cells treated with TNF-alpha for 24 h. Northern blot analysis of the beta-actin gene demonstrated that the cellular level of beta-actin mRNA was decreased at 6-12 h after exposure to TNF-alpha. However, there appeared to be no changes in cellular mRNA levels of beta-tubulin, fibronectin, laminin B1, laminin B2, and laminin binding protein genes after treatment with TNF-alpha. Nuclear run-on assays showed increased transcription of the low-density lipoprotein receptor gene, but not of the beta-actin gene. These data suggested that the TNF-alpha-induced inhibition of beta-actin gene expression was not due to altered transcription activity. The degradation rates of beta-actin, plasminogen activator inhibitor-1, and epidermal growth factor receptor mRNAs were examined in the presence of actinomycin D. beta-Actin mRNA was found to be specifically destabilized in TNF-alpha-treated HOME cells, while other mRNA species were not. Coadministration of cycloheximide blocked the TNF-alpha-induced degradation of beta-actin mRNA. The TNF-alpha-induced destabilization of beta-actin mRNA and rearrangement of actin filaments are discussed in relation to the morphological changes in human microvascular endothelial cells.
Similar articles
- Effects of tumor necrosis factor and epidermal growth factor on cell morphology, cell surface receptors, and the production of tissue inhibitor of metalloproteinases and IL-6 in human microvascular endothelial cells.
Mawatari M, Kohno K, Mizoguchi H, Matsuda T, Asoh K, Van Damme J, Welgus HG, Kuwano M. Mawatari M, et al. J Immunol. 1989 Sep 1;143(5):1619-27. J Immunol. 1989. PMID: 2547871 - Tumor necrosis factor decreases thrombin receptor expression in endothelial cells.
Yan W, Tiruppathi C, Qiao R, Lum H, Malik AB. Yan W, et al. J Cell Physiol. 1996 Mar;166(3):561-7. doi: 10.1002/(SICI)1097-4652(199603)166:3<561::AID-JCP10>3.0.CO;2-A. J Cell Physiol. 1996. PMID: 8600159 - Effects of interferon-gamma and tumor necrosis factor alpha on the expression of the genes encoding aggrecan, biglycan, and decorin core proteins in cultured human chondrocytes.
Dodge GR, Diaz A, Sanz-Rodriguez C, Reginato AM, Jimenez SA. Dodge GR, et al. Arthritis Rheum. 1998 Feb;41(2):274-83. doi: 10.1002/1529-0131(199802)41:2<274::AID-ART11>3.0.CO;2-Z. Arthritis Rheum. 1998. PMID: 9485085 Review. - Androgen regulates gene expression of cytoskeletal proteins in adult rat motoneurons.
Matsumoto A, Arai Y, Urano A, Hyodo S. Matsumoto A, et al. Horm Behav. 1994 Dec;28(4):357-66. doi: 10.1006/hbeh.1994.1032. Horm Behav. 1994. PMID: 7729804 Review.
Cited by
- Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis.
Yoshida S, Ono M, Shono T, Izumi H, Ishibashi T, Suzuki H, Kuwano M. Yoshida S, et al. Mol Cell Biol. 1997 Jul;17(7):4015-23. doi: 10.1128/MCB.17.7.4015. Mol Cell Biol. 1997. PMID: 9199336 Free PMC article. - Regulation of Epithelial and Endothelial Barriers by Molecular Chaperones.
Lechuga S, Marino-Melendez A, Naydenov NG, Zafar A, Braga-Neto MB, Ivanov AI. Lechuga S, et al. Cells. 2024 Feb 21;13(5):370. doi: 10.3390/cells13050370. Cells. 2024. PMID: 38474334 Free PMC article. Review. - TNF-α induces dyscohesion of epithelial cells. Association with disassembly of actin filaments.
Tabibzadeh S, Kong QF, Kapur S, Leffers H, Ridley A, Aktories K, Celis JE. Tabibzadeh S, et al. Endocrine. 1995 Aug;3(8):549-56. doi: 10.1007/BF02953018. Endocrine. 1995. PMID: 21153131 - Involvement of the transcription factor NF-kappaB in tubular morphogenesis of human microvascular endothelial cells by oxidative stress.
Shono T, Ono M, Izumi H, Jimi SI, Matsushima K, Okamoto T, Kohno K, Kuwano M. Shono T, et al. Mol Cell Biol. 1996 Aug;16(8):4231-9. doi: 10.1128/MCB.16.8.4231. Mol Cell Biol. 1996. PMID: 8754823 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials