Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10 - PubMed (original) (raw)
Comparative Study
. 1993 Sep 15;151(6):3206-14.
Affiliations
- PMID: 8376776
Comparative Study
Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10
N Ghildyal et al. J Immunol. 1993.
Abstract
BALB/cJ mouse mast cells derived by culturing bone marrow progenitor cells in WEHI-3 cell-conditioned medium (BMMCW) do not contain mouse mast cell protease 2 (mMCP-2) mRNA, but these cells can be induced to express this transcript after exposure to rIL-10. To study the translation and granule accumulation of mMCP-2 in rIL-10-treated BMMC (BMMCW+IL-10), a rabbit antibody was developed to a synthetic peptide that corresponds to the novel amino acid sequence in mMCP-2 at residues 56 to 71. After affinity purification, this antibody, anti-mMCP-2(56-71) IgG, reacted in SDS-PAGE/immunoblots against a 28-kDa protein in BMMCW+IL-10 that had the N-terminal amino acid sequence of mMCP-2. As assessed immunohistochemically, mMCP-2 protein accumulated in the secretory granules of Kirsten sarcoma virus-immortalized mouse mast cells, BMMCW+IL-10, and the mucosal mast cells present in the jejunum of Trichinella spiralis-infected BALB/cJ mice. Time course analyses of the induction of mMCP-2 mRNA and protein in BMMCW+IL-10 revealed that these cells contain a high steady-state level of mMCP-2 mRNA 24 h after their exposure to rIL-10. Although a small amount of immunodetectable mMCP-2 protein is present in the cells treated for 24 h, large amounts of this protease are not obtained until 7 days of treatment of the cells with rIL-10. Time course analyses of the loss of mMCP-2 mRNA and protein in BMMCW+IL-10 revealed that the steady-state level of mMCP-2 mRNA decreased dramatically 24 h after rIL-10 was removed from the culture medium, but that the level of mMCP-2 protein did not decline measurably until day 5 of culture. The fact that the steady-state levels of mMCP-2 mRNA and protein in BMMC can both be reversibly altered by culturing these mast cells in the presence and absence of rIL-10 suggests that the phenotype of mast cells is not fixed. Rather, it is in a dynamic state regulated by the cytokine network to which mast cells are exposed in their different microenvironments.
Similar articles
- IL-10 induces transcription of the gene for mouse mast cell protease-1, a serine protease preferentially expressed in mucosal mast cells of Trichinella spiralis-infected mice.
Ghildyal N, McNeil HP, Stechschulte S, Austen KF, Silberstein D, Gurish MF, Somerville LL, Stevens RL. Ghildyal N, et al. J Immunol. 1992 Sep 15;149(6):2123-9. J Immunol. 1992. PMID: 1517575 - Translation and granule localization of mouse mast cell protease-5. Immunodetection with specific antipeptide Ig.
McNeil HP, Frenkel DP, Austen KF, Friend DS, Stevens RL. McNeil HP, et al. J Immunol. 1992 Oct 1;149(7):2466-72. J Immunol. 1992. PMID: 1527387 - Lack of expression of the tryptase mouse mast cell protease 7 in mast cells of the C57BL/6J mouse.
Ghildyal N, Friend DS, Freelund R, Austen KF, McNeil HP, Schiller V, Stevens RL. Ghildyal N, et al. J Immunol. 1994 Sep 15;153(6):2624-30. J Immunol. 1994. PMID: 8077672 - Neutral proteases of mouse mast cells.
Serafin WE, Reynolds DS. Serafin WE, et al. Monogr Allergy. 1990;27:31-50. Monogr Allergy. 1990. PMID: 2084537 Review. No abstract available. - Granule chymases and the characterization of mast cell phenotype and function in rat and mouse.
Miller HR, Huntley JF, Newlands GF, Irvine J. Miller HR, et al. Monogr Allergy. 1990;27:1-30. Monogr Allergy. 1990. PMID: 2084533 Review. No abstract available.
Cited by
- IL-10 Neutralization Attenuates Mast Cell Responses in a Murine Model of Experimental Food Allergy.
Krajewski D, Ranjitkar S, Tedeschi C, Perez NM, Jordan N, Mire M, Schneider SS, Mathias CB. Krajewski D, et al. Immunohorizons. 2024 Jun 1;8(6):431-441. doi: 10.4049/immunohorizons.2400002. Immunohorizons. 2024. PMID: 38888412 Free PMC article. - Mucosal Mast Cells as Key Effector Cells in Food Allergies.
Nakano N, Kitaura J. Nakano N, et al. Cells. 2022 Jan 19;11(3):329. doi: 10.3390/cells11030329. Cells. 2022. PMID: 35159139 Free PMC article. Review. - Regulation and function of mast cell proteases in inflammation.
Huang C, Sali A, Stevens RL. Huang C, et al. J Clin Immunol. 1998 May;18(3):169-83. doi: 10.1023/a:1020574820797. J Clin Immunol. 1998. PMID: 9624576 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Research Materials