The Epstein-Barr virus nuclear antigen 2 interacts with an EBNA2 responsive cis-element of the terminal protein 1 gene promoter - PubMed (original) (raw)

The Epstein-Barr virus nuclear antigen 2 interacts with an EBNA2 responsive cis-element of the terminal protein 1 gene promoter

U Zimber-Strobl et al. EMBO J. 1993 Jan.

Abstract

The Epstein-Barr virus protein EBNA2 acts as a transcriptional activator of cellular and viral genes and plays a crucial role in the immortalization of human primary B-cells by EBV. We have shown previously that EBNA2 transactivates the promoters of the latent membrane antigens LMP, TP1 and TP2. The promoter of the TP1 gene was chosen as a model system to study the molecular mechanism of EBNA2 mediated transactivation. To identify an EBNA2 dependent cis-acting element, various TP1 promoter-reporter gene constructs were transfected in the absence and presence of an EBNA2 expression vector into the established B-cell line BL41-P3HR1. We were able to delineate an 81 bp EBNA2 responsive region between -258 and -177 relative to the TP1 RNA start site. The element worked in either orientation and could mediate EBNA2 dependent transactivation on a heterologous promoter. Electrophoretic mobility shift assays revealed three specific protein-DNA complexes formed with sequences of the EBNA2 responsive element. Two of these were not cell type specific, but the third was detected only in EBNA2 positive cell extracts. Gel-shift analysis in the presence of EBNA2 specific monoclonal antibodies revealed that EBNA2 is a component of the third complex. Thus, these experiments demonstrate that EBNA2 interacts with an EBNA2 responsive cis-element of the TP1 promoter.

PubMed Disclaimer

Cited by

The Epstein-Barr virus immortalizing protein EBNA-2 is targeted to DNA by a cellular enhancer-binding protein.
Ling PD, Rawlins DR, Hayward SD. Ling PD, et al. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9237-41. doi: 10.1073/pnas.90.20.9237. Proc Natl Acad Sci U S A. 1993. PMID: 8415684 Free PMC article.
The Epstein-Barr virus BamHI C promoter is not essential for B cell immortalization in vitro, but it greatly enhances B cell growth transformation.
Tierney RJ, Nagra J, Rowe M, Bell AI, Rickinson AB. Tierney RJ, et al. J Virol. 2015 Mar;89(5):2483-93. doi: 10.1128/JVI.03300-14. Epub 2014 Dec 24. J Virol. 2015. PMID: 25540367 Free PMC article.
Immortalization of human B lymphocytes by a plasmid containing 71 kilobase pairs of Epstein-Barr virus DNA.
Kempkes B, Pich D, Zeidler R, Sugden B, Hammerschmidt W. Kempkes B, et al. J Virol. 1995 Jan;69(1):231-8. doi: 10.1128/JVI.69.1.231-238.1995. J Virol. 1995. PMID: 7983714 Free PMC article.
The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE.
Tong X, Drapkin R, Yalamanchili R, Mosialos G, Kieff E. Tong X, et al. Mol Cell Biol. 1995 Sep;15(9):4735-44. doi: 10.1128/MCB.15.9.4735. Mol Cell Biol. 1995. PMID: 7651391 Free PMC article.

References

1. J Virol. 1967 Oct;1(5):1045-51 - PubMed
1. J Virol. 1991 Nov;65(11):5880-5 - PubMed
1. J Mol Biol. 1976 Apr 15;102(3):511-30 - PubMed
1. Proc Natl Acad Sci U S A. 1978 May;75(5):2458-62 - PubMed
1. IARC Sci Publ. 1985;(60):309-18 - PubMed

The Epstein-Barr virus nuclear antigen 2 interacts with an EBNA2 responsive cis-element of the terminal protein 1 gene promoter - PubMed (original) (raw)