A pH induced two-dimensional crystal of membrane-bound Na+,K(+)-ATPase of dog kidney - PubMed (original) (raw)
A pH induced two-dimensional crystal of membrane-bound Na+,K(+)-ATPase of dog kidney
Y Tahara et al. FEBS Lett. 1993.
Free article
Abstract
Two-dimensional crystals of membrane-bound Na+,K(+)-ATPase were formed in acidic media and their qualities were investigated by electron cryo-microscopy as well as by conventional electron microscopy. At pH 4.8 in sodium citrate buffer, the best crystallization condition, more than 80% of membranes formed crystals. The high ratio allowed high-resolution images to be taken by electron cryo-microscopy. Image processing revealed that they had unique lattice constants (a = 108.7 A, b = 66.2 A, gamma = 104.2 degrees) and had few defects in the crystalline arrays. The reconstituted Fourier map of the ice-embedded crystal showed that there are two high contrast parts in one unit cell.
Similar articles
- Reconstitution of detergent-solubilized Na,K-ATPase and formation of two-dimensional crystals.
Mohraz M. Mohraz M. J Struct Biol. 1999 Mar;125(1):76-85. doi: 10.1006/jsbi.1998.4067. J Struct Biol. 1999. PMID: 10196118 - Molecular imaging of Na+,K(+)-ATPase in purified kidney membranes.
Paul JK, Nettikadan SR, Ganjeizadeh M, Yamaguchi M, Takeyasu K. Paul JK, et al. FEBS Lett. 1994 Jun 13;346(2-3):289-94. doi: 10.1016/0014-5793(94)00448-x. FEBS Lett. 1994. PMID: 8013648 - Further characterization of the 3-dimensional crystals of detergent-solubilized (Na+,K+)-ATPase from pig kidney.
Varga S, Szabolcs M. Varga S, et al. Acta Physiol Hung. 1994;82(2):139-52. Acta Physiol Hung. 1994. PMID: 7887174 - Two-dimensional crystals and three-dimensional structure of Na,K-ATPase analyzed by electron microscopy.
Maunsbach AB, Skriver E, Hebert H. Maunsbach AB, et al. Soc Gen Physiol Ser. 1991;46:159-72. Soc Gen Physiol Ser. 1991. PMID: 1653976 Review. No abstract available.
Cited by
- Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy.
Ohi M, Li Y, Cheng Y, Walz T. Ohi M, et al. Biol Proced Online. 2004;6:23-34. doi: 10.1251/bpo70. Epub 2004 Mar 19. Biol Proced Online. 2004. PMID: 15103397 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources