A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor - PubMed (original) (raw)

A bacterially expressed single-chain Fv construct from the 2B4 T-cell receptor

I Kurucz et al. Proc Natl Acad Sci U S A. 1993.

Abstract

A single-chain Fv construct of the 2B4 T-cell receptor has been made and expressed in Escherichia coli as bacterial inclusion bodies. After solubilization in 6 M guanidine hydrochloride and formation of mixed disulfides with glutathione, the protein was refolded by diluting out the denaturant and allowing intramolecular disulfide bridges to form by disulfide exchange. Approximately 65-100 mg of refolded protein was obtained from 1 liter of bacterial culture, an appreciable fraction of which was monomeric in nondenaturing solvents. This protein bound to three monoclonal antibodies specific for allotypic or idiotypic determinants on the native 2B4 variable region but did not bind several other anti-T-cell-receptor monoclonal antibodies that lacked such specificity. These experiments show that T-cell-receptor variable regions, like the V regions of antibodies, can form a well-behaved single-chain Fv molecule and provide large amounts of recombinant single-chain Fv T-cell receptor that can be used to study T-cell function.

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References

1. 1. Science. 1990 Aug 10;249(4969):677-9 - PubMed
2. 1. J Mol Biol. 1992 Apr 20;224(4):885-90 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6972-6 - PubMed
4. 1. Nature. 1984 Mar 8-14;308(5955):153-8 - PubMed
5. 1. Nature. 1984 May 24-30;309(5966):322-6 - PubMed

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