Sox-4, an Sry-like HMG box protein, is a transcriptional activator in lymphocytes - PubMed (original) (raw)
Sox-4, an Sry-like HMG box protein, is a transcriptional activator in lymphocytes
M van de Wetering et al. EMBO J. 1993 Oct.
Abstract
Previous studies in lymphocytes have described two DNA-binding HMG box proteins, TCF-1 and LEF-1, with affinity for the A/TA/TCAAAG motif found in several T cell-specific enhancers. Evaluation of cotransfection experiments in non-T cells and the observed inactivity of an AACAAAG concatamer in the TCF-1/LEF-1-expressing T cell line BW5147, led us to conclude that these two proteins did not mediate the observed enhancer effect. We therefore searched for additional HMG box proteins. By a PCR-aided strategy, we cloned Sox-4, a gene with homology to the HMG box region of the sex determining gene SRY. Sox-4 was expressed in T and pre-B lymphocyte lines and in the murine thymus. Significantly, BW5147 T cells did not express Sox-4. Recombinant Sox-4 bound with high affinity (Kd 3 x 10(-11) M) to the minor groove of the AACAAAG motif, most likely contacting all seven base pairs. In contrast with observations on TCF-1 and LEF-1, cotransfection with Sox-4 unveiled a transactivating capacity, which mapped to its serine-rich C terminus. This region remained functional upon grafting onto a GAL4 DNA-binding domain. Sox-4 is thus the first 'classical' transcription factor in the Sox gene family with separable DNA-binding and transactivation domains. Our observations indicate that a detailed understanding of T cell-specific gene control must integrate the concerted activity of at least three tissue-specific HMG box genes.
Similar articles
- Solution structure of the sequence-specific HMG box of the lymphocyte transcriptional activator Sox-4.
van Houte LP, Chuprina VP, van der Wetering M, Boelens R, Kaptein R, Clevers H. van Houte LP, et al. J Biol Chem. 1995 Dec 22;270(51):30516-24. doi: 10.1074/jbc.270.51.30516. J Biol Chem. 1995. PMID: 8530483 - Sequence-specific interaction of the HMG box proteins TCF-1 and SRY occurs within the minor groove of a Watson-Crick double helix.
van de Wetering M, Clevers H. van de Wetering M, et al. EMBO J. 1992 Aug;11(8):3039-44. doi: 10.1002/j.1460-2075.1992.tb05374.x. EMBO J. 1992. PMID: 1639073 Free PMC article. - A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein.
Takamatsu N, Kanda H, Tsuchiya I, Yamada S, Ito M, Kabeno S, Shiba T, Yamashita S. Takamatsu N, et al. Mol Cell Biol. 1995 Jul;15(7):3759-66. doi: 10.1128/MCB.15.7.3759. Mol Cell Biol. 1995. PMID: 7791783 Free PMC article. - HMG box containing transcription factors in lymphocyte differentiation.
Schilham MW, Clevers H. Schilham MW, et al. Semin Immunol. 1998 Apr;10(2):127-32. doi: 10.1006/smim.1998.0114. Semin Immunol. 1998. PMID: 9618758 Review. - Floppy SOX: mutual induced fit in hmg (high-mobility group) box-DNA recognition.
Weiss MA. Weiss MA. Mol Endocrinol. 2001 Mar;15(3):353-62. doi: 10.1210/mend.15.3.0617. Mol Endocrinol. 2001. PMID: 11222737 Review.
Cited by
- Cell of origin epigenetic priming determines susceptibility to Tet2 mutation.
Schiroli G, Kartha V, Duarte FM, Kristiansen TA, Mayerhofer C, Shrestha R, Earl A, Hu Y, Tay T, Rhee C, Buenrostro JD, Scadden DT. Schiroli G, et al. Nat Commun. 2024 May 21;15(1):4325. doi: 10.1038/s41467-024-48508-6. Nat Commun. 2024. PMID: 38773071 Free PMC article. - Landscape of Constitutional SOX4 Variation in Human Disorders.
Grippa M, Graziano C. Grippa M, et al. Genes (Basel). 2024 Jan 25;15(2):158. doi: 10.3390/genes15020158. Genes (Basel). 2024. PMID: 38397148 Free PMC article. Review. - SOX on tumors, a comfort or a constraint?
Jiang J, Wang Y, Sun M, Luo X, Zhang Z, Wang Y, Li S, Hu D, Zhang J, Wu Z, Chen X, Zhang B, Xu X, Wang S, Xu S, Huang W, Xia L. Jiang J, et al. Cell Death Discov. 2024 Feb 9;10(1):67. doi: 10.1038/s41420-024-01834-6. Cell Death Discov. 2024. PMID: 38331879 Free PMC article. Review. - Genome-Wide Identification, Evolutionary and Mutational Analysis of the Buffalo Sox Gene Family.
Abdullah M, Rehman MS, Rehman MSN, AlKahtane AA, Al-Hazani TM, Hassan FU, Rehman SU. Abdullah M, et al. Animals (Basel). 2023 Jul 8;13(14):2246. doi: 10.3390/ani13142246. Animals (Basel). 2023. PMID: 37508024 Free PMC article. - Thymic tuft cells: potential "regulators" of non-mucosal tissue development and immune response.
Sun J, Li MX, Xie YM, Zhang YR, Chai YR. Sun J, et al. Immunol Res. 2023 Aug;71(4):554-564. doi: 10.1007/s12026-023-09372-6. Epub 2023 Mar 24. Immunol Res. 2023. PMID: 36961668 Free PMC article. Review.
References
- EMBO J. 1992 Dec;11(13):4961-8 - PubMed
- EMBO J. 1992 Dec;11(12):4497-506 - PubMed
- Nucleic Acids Res. 1993 Apr 11;21(7):1669 - PubMed
- Nucleic Acids Res. 1993 Apr 25;21(8):2009 - PubMed
- Nucleic Acids Res. 1993 May 25;21(10):2493-501 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous