Protein transport to the dendritic plasma membrane of cultured neurons is regulated by rab8p - PubMed (original) (raw)

Protein transport to the dendritic plasma membrane of cultured neurons is regulated by rab8p

L A Huber et al. J Cell Biol. 1993 Oct.

Abstract

In the companion paper (Huber, L. A., S. W. Pimplikar, R. G. Parton, H. Virta, M. Zerial, and K. Simons. J. Cell Biol. 123:35-45) we reported that the small GTPase rab8p is involved in transport from the TGN to the basolateral plasma membrane in epithelia. In the present work we investigated the localization and function of rab8p in polarized hippocampal neurons. By immunofluorescence microscopy we found that rab8p localized preferentially in the somatodendritic domain, and was excluded from the axon. Double-labeling immunofluorescence showed that some of the rab8p co-localized in the dendrites with the Semliki Forest Virus glycoprotein E2 (SFV-E2). An antisense oligonucleotide approach was used to investigate the role of rab8p in dendritic transport of newly synthesized viral glycoproteins. Antisense oligonucleotides corresponding to the initiation region of the rab8 coding sequence were added to the cultured neurons for four days. This treatment resulted in a significant decrease in cellular levels of rab8p and transport of SFV-E2 from the cell body to the dendrites was significantly reduced. However, no effect was observed on axonal transport of influenza HA. From these results we conclude that rab8p is involved in transport of proteins to the dendritic surface in neurons.

PubMed Disclaimer

Similar articles

Cited by

References

    1. J Neurosci. 1984 Aug;4(8):1954-65 - PubMed
    1. J Membr Biol. 1990 Jul;117(1):1-10 - PubMed
    1. Cell. 1980 May;20(1):45-54 - PubMed
    1. J Cell Biol. 1993 Oct;123(1):35-45 - PubMed
    1. EMBO J. 1985 Oct;4(10):2475-85 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources