TGF beta suppresses casein synthesis in mouse mammary explants and may play a role in controlling milk levels during pregnancy - PubMed (original) (raw)

TGF beta suppresses casein synthesis in mouse mammary explants and may play a role in controlling milk levels during pregnancy

S D Robinson et al. J Cell Biol. 1993 Jan.

Abstract

Mammary explants from 14-15-d-pregnant mice synthesize and secrete milk proteins in culture in response to insulin, hydrocortisone, and prolactin. Here we demonstrate that transforming growth factor beta (TGF beta) treatment suppresses, in a dose dependent and reversible manner, the ability of explants to synthesize and secrete milk caseins. TGF beta does not affect the level of casein mRNA within explants but inhibits casein synthesis posttranscriptionally. We also show increased expression of TGF beta 2 and TGF beta 3 in intact mammary gland as pregnancy progresses, with reduced expression of all three TGF betas at the onset of lactation. These findings suggest that endogenously produced TGF beta may limit the accumulation of milk caseins that are produced in the mammary gland during pregnancy.

PubMed Disclaimer

References

1. 1. Cancer Treat Res. 1988;40:1-24 -PubMed
2. 1. Mol Cell Biol. 1990 Aug;10(8):4027-34 -PubMed
3. 1. Science. 1957 Oct 25;126(3278):842-3 -PubMed
4. 1. Development. 1991 Nov;113(3):867-78 -PubMed
5. 1. J Dairy Sci. 1991 Aug;74(8):2788-800 -PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PMC
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database