Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro - PubMed (original) (raw)
Comparative Study
Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro
N K Pryer et al. J Cell Biol. 1993 Feb.
Abstract
The SEC13 gene of Saccharomyces cerevisiae is required in vesicle biogenesis at a step before or concurrent with the release of transport vesicles from the ER membrane. SEC13 encodes a 33-kD protein with sequence homology to a series of conserved internal repeat motifs found in beta subunits of heterotrimeric G proteins. The product of this gene, Sec13p, is a cytosolic protein peripherally associated with membranes. We developed a cell-free Sec13p-dependent vesicle formation reaction. Sec13p-depleted membranes and cytosol fractions were generated by urea treatment of membranes and affinity depletion of a Sec13p-dihydrofolate reductase fusion protein, respectively. These fractions were unable to support vesicle formation from the ER unless cytosol containing Sec13p was added. Cytosolic Sec13p fractionated by gel filtration as a large complex of about 700 kD. Fractions containing the Sec13p complex restored activity to the Sec13p- dependent vesicle formation reaction. Expression of SEC13 on a multicopy plasmid resulted in overproduction of a monomeric form of Sec13p, suggesting that another member of the complex becomes limiting when Sec13p is overproduced. Overproduced, monomeric Sec13p was inactive in the Sec13p-dependent vesicle formation assay.
Similar articles
- The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins.
Salama NR, Yeung T, Schekman RW. Salama NR, et al. EMBO J. 1993 Nov;12(11):4073-82. doi: 10.1002/j.1460-2075.1993.tb06091.x. EMBO J. 1993. PMID: 8223424 Free PMC article. - The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus.
Tang BL, Peter F, Krijnse-Locker J, Low SH, Griffiths G, Hong W. Tang BL, et al. Mol Cell Biol. 1997 Jan;17(1):256-66. doi: 10.1128/MCB.17.1.256. Mol Cell Biol. 1997. PMID: 8972206 Free PMC article. - Sec31 encodes an essential component of the COPII coat required for transport vesicle budding from the endoplasmic reticulum.
Salama NR, Chuang JS, Schekman RW. Salama NR, et al. Mol Biol Cell. 1997 Feb;8(2):205-17. doi: 10.1091/mbc.8.2.205. Mol Biol Cell. 1997. PMID: 9190202 Free PMC article. - COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
Barlowe C, Orci L, Yeung T, Hosobuchi M, Hamamoto S, Salama N, Rexach MF, Ravazzola M, Amherdt M, Schekman R. Barlowe C, et al. Cell. 1994 Jun 17;77(6):895-907. doi: 10.1016/0092-8674(94)90138-4. Cell. 1994. PMID: 8004676 - Membrane protein insertion into the endoplasmic reticulum--another channel tunnel?
High S. High S. Bioessays. 1992 Aug;14(8):535-40. doi: 10.1002/bies.950140807. Bioessays. 1992. PMID: 1365907 Review.
Cited by
- COPII cage assembly factor Sec13 integrates information flow regulating endomembrane function in response to human variation.
Anglès F, Gupta V, Wang C, Balch WE. Anglès F, et al. Sci Rep. 2024 May 3;14(1):10160. doi: 10.1038/s41598-024-60687-2. Sci Rep. 2024. PMID: 38698045 Free PMC article. - Molecular basis of the inositol deacylase PGAP1 involved in quality control of GPI-AP biogenesis.
Hong J, Li T, Chao Y, Xu Y, Zhu Z, Zhou Z, Gu W, Qu Q, Li D. Hong J, et al. Nat Commun. 2024 Jan 2;15(1):8. doi: 10.1038/s41467-023-44568-2. Nat Commun. 2024. PMID: 38167496 Free PMC article. - The ER-Golgi transport of influenza virus through NS1-Sec13 association during virus replication.
Chua SCJH, Cui J, Sachaphibulkij K, Tan ISL, Tan HQ, Lim HM, Engelberg D, Lim LHK. Chua SCJH, et al. Microbiol Spectr. 2024 Jan 11;12(1):e0260923. doi: 10.1128/spectrum.02609-23. Epub 2023 Dec 1. Microbiol Spectr. 2024. PMID: 38038453 Free PMC article. - Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.
Liu Z, Yan M, Lei W, Jiang R, Dai W, Chen J, Wang C, Li L, Wu M, Nian X, Li D, Sun D, Lv X, Wang C, Xie C, Yao L, Wu C, Hu J, Xiao N, Mo W, Wang Z, Zhang L. Liu Z, et al. J Clin Invest. 2022 Apr 1;132(7):e155096. doi: 10.1172/JCI155096. J Clin Invest. 2022. PMID: 35143418 Free PMC article. - Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis.
Seok HY, Bae H, Kim T, Mehdi SMM, Nguyen LV, Lee SY, Moon YH. Seok HY, et al. Int J Mol Sci. 2021 Apr 29;22(9):4738. doi: 10.3390/ijms22094738. Int J Mol Sci. 2021. PMID: 33947021 Free PMC article.
References
- J Biol Chem. 1992 Jun 15;267(17):12106-15 - PubMed
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- Cell. 1988 Jul 29;54(3):335-44 - PubMed
- Methods Enzymol. 1990;185:144-61 - PubMed
- Mol Cell Biol. 1990 Jul;10(7):3405-14 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases