Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonas reinhardtii - PubMed (original) (raw)

. 1993 Jan;236(2-3):409-14.

doi: 10.1007/BF00277141.

Affiliations

• PMID: 8437585
• DOI: 10.1007/BF00277141

Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonas reinhardtii

F Dürrenberger et al. Mol Gen Genet. 1993 Jan.

Abstract

The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI recognition sequence is 19-24 bp in size and is located asymmetrically around the intron insertion site. Screening of natural variants of the I-CreI recognition sequence indicates that the I-CreI endonuclease tolerates single and even multiple base changes within its recognition sequence.

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References

1. 1. DNA. 1984 Oct;3(5):347-57 - PubMed
2. 1. Nucleic Acids Res. 1986 May 12;14(9):3827-39 - PubMed
3. 1. Cell. 1989 Feb 10;56(3):431-41 - PubMed
4. 1. J Biol Chem. 1984 Apr 25;259(8):5173-81 - PubMed
5. 1. Mol Cell Biol. 1992 Feb;12(2):716-23 - PubMed

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