Cell surface expression of lysosome-associated membrane proteins (LAMPs) in scleroderma: relationship of lamp2 to disease duration, anti-Sc170 antibodies, serum interleukin-8, and soluble interleukin-2 receptor levels - PubMed (original) (raw)
Cell surface expression of lysosome-associated membrane proteins (LAMPs) in scleroderma: relationship of lamp2 to disease duration, anti-Sc170 antibodies, serum interleukin-8, and soluble interleukin-2 receptor levels
R F Holcombe et al. Clin Immunol Immunopathol. 1993 Apr.
Abstract
Lysosome-associated membrane proteins (LAMPs) are integral transmembrane proteins densely expressed on lysosomes which can be shuttled to the plasma membrane during cell activation. The objective of this study was to examine the expression of LAMPs on the cell surface of peripheral blood mononuclear cells (PBMCs) derived from patients with scleroderma. Heparinized blood was obtained from 23 patients with scleroderma and 15 healthy controls and PBMCs were isolated via a Ficoll gradient. Cells were stained with monoclonal antibodies directed against two of the major LAMPs, lamp1 and lamp2, and after subsequent staining with a fluorescent second antibody, were analyzed by flow cytometry. Two -and three-color immunofluorescence was utilized to define the phenotype of LAMP+ cells. The proportion of PBMCs expressing lamp2 on the cell surface was significantly elevated in patients with scleroderma (2.21 +/- 0.38%) compared to controls (1.14 +/- 0.27%; P < 0.05). Multivariate analysis of patient subgroups indicated that the significant factors contributing to higher levels in patients were shorter duration of disease (P < 0.01) and greater functional disability related to disease manifestations (P < 0.01). Patients with anti-Scl70 antibodies had the highest levels of cell surface lamp2 expression (4.19 +/- 0.90%; P < 0.0005). The degree of cell surface lamp2 expression correlated with the level of sIL2R in 19 scleroderma patients (r = 0.48; P < 0.05). Serum IL4 and IL6 did not correlate with cell surface LAMPs or sIL2R. Five of 19 patients had detectable serum levels of interleukin-8 (IL8). These patients had significantly higher cell surface lamp2 expression than those with no detectable IL8 (3.76 +/- 0.48% vs 1.44 +/- 0.39%; P < 0.01). Extended phenotyping revealed that > 85% of lamp2+ cells expressed the B-cell antigen CD19. Cell surface lamp2 expression correlates with clinical and laboratory parameters in scleroderma patients and may reflect immune system activation. Additionally, this is the first report describing an elevation of serum IL8 in an autoimmune or collagen-vascular disease.
Similar articles
- Correlation of serum interleukin-8 and cell surface lysosome-associated membrane protein expression with clinical disease activity in systemic lupus erythematosus.
Holcombe RF, Baethge BA, Wolf RE, Betzing KW, Stewart RM, Hall VC, Fukuda M. Holcombe RF, et al. Lupus. 1994 Apr;3(2):97-102. doi: 10.1177/096120339400300207. Lupus. 1994. PMID: 7920621 - Cell surface expression of lysosome-associated membrane protein-2 (lamp2) and CD63 as markers of in vivo platelet activation in malignancy.
Kannan K, Divers SG, Lurie AA, Chervenak R, Fukuda M, Holcombe RF. Kannan K, et al. Eur J Haematol. 1995 Sep;55(3):145-51. doi: 10.1111/j.1600-0609.1995.tb00242.x. Eur J Haematol. 1995. PMID: 7672086 - LAMPs: Shedding light on cancer biology.
Alessandrini F, Pezzè L, Ciribilli Y. Alessandrini F, et al. Semin Oncol. 2017 Aug;44(4):239-253. doi: 10.1053/j.seminoncol.2017.10.013. Epub 2017 Nov 4. Semin Oncol. 2017. PMID: 29526252 Review. - OMIP-047: High-Dimensional phenotypic characterization of B cells.
Liechti T, Günthard HF, Trkola A. Liechti T, et al. Cytometry A. 2018 Jun;93(6):592-596. doi: 10.1002/cyto.a.23488. Epub 2018 May 21. Cytometry A. 2018. PMID: 29782066 Free PMC article. Review. No abstract available.
Cited by
- Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells.
Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H. Tian Y, et al. Clin Proteomics. 2015 Apr 11;12(1):11. doi: 10.1186/s12014-015-9083-8. eCollection 2015. Clin Proteomics. 2015. PMID: 25987888 Free PMC article. - Proteomic analysis of temporally stimulated ovarian cancer cells for biomarker discovery.
Marzinke MA, Choi CH, Chen L, Shih IeM, Chan DW, Zhang H. Marzinke MA, et al. Mol Cell Proteomics. 2013 Feb;12(2):356-68. doi: 10.1074/mcp.M112.019521. Epub 2012 Nov 19. Mol Cell Proteomics. 2013. PMID: 23172893 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous