Multiple pseudouridine synthase activities for small nuclear RNAs - PubMed (original) (raw)
Multiple pseudouridine synthase activities for small nuclear RNAs
J R Patton. Biochem J. 1993.
Abstract
The formation of pseudouridine (psi) in human U1, U2 and U5 small nuclear RNAs (snRNAs) was investigated using HeLa cell extracts. Unmodified snRNAs were synthesized in vitro and the extent of psi formation was determined after incubation in cell extracts. The formation of psi on labelled substrates was monitored in the presence of 5-fluorouracil (5-FU)-containing snRNAs as inhibitors of psi formation. The conversion of uridine to psi was inhibited only when the cognate 5-FU-containing inhibitor snRNA was included in the reaction. For example, 5-FU-containing U1 RNA inhibited psi formation in unmodified U1 RNA, but not in (unmodified) U2 or U5 RNAs. The results suggest that there are at least three activities that form psi in these snRNAs. The 5-FU-containing RNAs were stable during incubation in the cell extracts. A 12-fold molar excess of unlabelled U1 RNA did not inhibit psi formation on a labelled U1 RNA substrate, whereas a 3-fold molar excess of 5-FU-containing U1 RNA nearly abolished psi formation on the U1 substrate. The fact that 5-FU-containing snRNAs are potent inhibitors of psi formation in these pre-mRNA splicing cofactors raises the possibility that this is related to the cytotoxicity of fluoropyrimidines in cancer chemotherapy.
Similar articles
- Modification of human U4 RNA requires U6 RNA and multiple pseudouridine synthases.
Zerby DB, Patton JR. Zerby DB, et al. Nucleic Acids Res. 1997 Dec 1;25(23):4808-15. doi: 10.1093/nar/25.23.4808. Nucleic Acids Res. 1997. PMID: 9365261 Free PMC article. - Formation of pseudouridine in U5 small nuclear RNA.
Patton JR. Patton JR. Biochemistry. 1994 Aug 30;33(34):10423-7. doi: 10.1021/bi00200a025. Biochemistry. 1994. PMID: 8068680 - Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs: unique modifications are found in regions involved in snRNA-snRNA interactions.
Patton JR, Padgett RW. Patton JR, et al. BMC Mol Biol. 2005 Oct 19;6:20. doi: 10.1186/1471-2199-6-20. BMC Mol Biol. 2005. PMID: 16236171 Free PMC article. - Pseudouridine formation in small nuclear RNAs.
Patton JR. Patton JR. Biochimie. 1994;76(12):1129-32. doi: 10.1016/0300-9084(94)90041-8. Biochimie. 1994. PMID: 7748947 Review. - Pseudouridine in RNA: what, where, how, and why.
Charette M, Gray MW. Charette M, et al. IUBMB Life. 2000 May;49(5):341-51. doi: 10.1080/152165400410182. IUBMB Life. 2000. PMID: 10902565 Review.
Cited by
- RNA modification: mechanisms and therapeutic targets.
Qiu L, Jing Q, Li Y, Han J. Qiu L, et al. Mol Biomed. 2023 Aug 24;4(1):25. doi: 10.1186/s43556-023-00139-x. Mol Biomed. 2023. PMID: 37612540 Free PMC article. Review. - Post-transcriptional pseudouridylation in mRNA as well as in some major types of noncoding RNAs.
Adachi H, De Zoysa MD, Yu YT. Adachi H, et al. Biochim Biophys Acta Gene Regul Mech. 2019 Mar;1862(3):230-239. doi: 10.1016/j.bbagrm.2018.11.002. Epub 2018 Nov 8. Biochim Biophys Acta Gene Regul Mech. 2019. PMID: 30414851 Free PMC article. Review. - The Role of Noncoding RNA Pseudouridylation in Nuclear Gene Expression Events.
Zhao Y, Dunker W, Yu YT, Karijolich J. Zhao Y, et al. Front Bioeng Biotechnol. 2018 Feb 8;6:8. doi: 10.3389/fbioe.2018.00008. eCollection 2018. Front Bioeng Biotechnol. 2018. PMID: 29473035 Free PMC article. Review. - Posttranscriptional RNA Pseudouridylation.
De Zoysa MD, Yu YT. De Zoysa MD, et al. Enzymes. 2017;41:151-167. doi: 10.1016/bs.enz.2017.02.001. Epub 2017 Mar 11. Enzymes. 2017. PMID: 28601221 Free PMC article. Review. - Insight into the mechanisms and functions of spliceosomal snRNA pseudouridylation.
Adachi H, Yu YT. Adachi H, et al. World J Biol Chem. 2014 Nov 26;5(4):398-408. doi: 10.4331/wjbc.v5.i4.398. World J Biol Chem. 2014. PMID: 25426264 Free PMC article. Review.
References
- Nucleic Acids Res. 1979 Apr;6(4):1571-81 - PubMed
- J Bacteriol. 1977 Apr;130(1):4-10 - PubMed
- J Biol Chem. 1982 Mar 25;257(6):3045-52 - PubMed
- Biochim Biophys Acta. 1982 Apr 26;697(1):31-40 - PubMed
- Adv Enzymol Relat Areas Mol Biol. 1983;54:58-119 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources