Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis - PubMed (original) (raw)
Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis
J P Morrissey et al. Mol Cell Biol. 1993 Apr.
Abstract
Subnuclear fractionation and coprecipitation by antibodies against the nucleolar protein NOP1 demonstrate that the essential Saccharomyces cerevisiae RNA snR30 is localized to the nucleolus. By using aminomethyl trimethyl-psoralen, snR30 can be cross-linked in vivo to 35S pre-rRNA. To determine whether snR30 has a role in rRNA processing, a conditional allele was constructed by replacing the authentic SNR30 promoter with the GAL10 promoter. Repression of snR30 synthesis results in a rapid depletion of snR30 and a progressive increase in cell doubling time. rRNA processing is disrupted during the depletion of snR30; mature 18S rRNA and its 20S precursor underaccumulate, and an aberrant 23S pre-rRNA intermediate can be detected. Initial results indicate that this 23S pre-rRNA is the same as the species detected on depletion of the small nucleolar RNA-associated proteins NOP1 and GAR1 and in an snr10 mutant strain. It was found that the 3' end of 23S pre-rRNA is located in the 3' region of ITS1 between cleavage sites A2 and B1 and not, as previously suggested, at the B1 site, snR30 is the fourth small nucleolar RNA shown to play a role in rRNA processing.
Similar articles
- snR30/U17 Small Nucleolar Ribonucleoprotein: A Critical Player during Ribosome Biogenesis.
Vos TJ, Kothe U. Vos TJ, et al. Cells. 2020 Sep 29;9(10):2195. doi: 10.3390/cells9102195. Cells. 2020. PMID: 33003357 Free PMC article. Review. - U17/snR30 is a ubiquitous snoRNA with two conserved sequence motifs essential for 18S rRNA production.
Atzorn V, Fragapane P, Kiss T. Atzorn V, et al. Mol Cell Biol. 2004 Feb;24(4):1769-78. doi: 10.1128/MCB.24.4.1769-1778.2004. Mol Cell Biol. 2004. PMID: 14749391 Free PMC article. - Base pairing between U3 small nucleolar RNA and the 5' end of 18S rRNA is required for pre-rRNA processing.
Sharma K, Tollervey D. Sharma K, et al. Mol Cell Biol. 1999 Sep;19(9):6012-9. doi: 10.1128/MCB.19.9.6012. Mol Cell Biol. 1999. PMID: 10454548 Free PMC article. - 18S rRNA processing requires base pairings of snR30 H/ACA snoRNA to eukaryote-specific 18S sequences.
Fayet-Lebaron E, Atzorn V, Henry Y, Kiss T. Fayet-Lebaron E, et al. EMBO J. 2009 May 6;28(9):1260-70. doi: 10.1038/emboj.2009.79. Epub 2009 Mar 26. EMBO J. 2009. PMID: 19322192 Free PMC article. - Small nucleolar RNA.
Gerbi SA. Gerbi SA. Biochem Cell Biol. 1995 Nov-Dec;73(11-12):845-58. doi: 10.1139/o95-092. Biochem Cell Biol. 1995. PMID: 8722000 Review.
Cited by
- Synergistic interaction network between the snR30 RNP, Utp23, and ribosomal RNA during ribosome synthesis.
Vos TJ, Kothe U. Vos TJ, et al. RNA Biol. 2022 Jan;19(1):764-773. doi: 10.1080/15476286.2022.2078092. RNA Biol. 2022. PMID: 35648701 Free PMC article. - Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast.
Jaafar M, Contreras J, Dominique C, Martín-Villanueva S, Capeyrou R, Vitali P, Rodríguez-Galán O, Velasco C, Humbert O, Watkins NJ, Villalobo E, Bohnsack KE, Bohnsack MT, Henry Y, Merhi RA, de la Cruz J, Henras AK. Jaafar M, et al. Nat Commun. 2021 Oct 22;12(1):6153. doi: 10.1038/s41467-021-26207-w. Nat Commun. 2021. PMID: 34686656 Free PMC article. - Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.
Baldini L, Charpentier B, Labialle S. Baldini L, et al. Noncoding RNA. 2021 May 6;7(2):30. doi: 10.3390/ncrna7020030. Noncoding RNA. 2021. PMID: 34066559 Free PMC article. Review. - snR30/U17 Small Nucleolar Ribonucleoprotein: A Critical Player during Ribosome Biogenesis.
Vos TJ, Kothe U. Vos TJ, et al. Cells. 2020 Sep 29;9(10):2195. doi: 10.3390/cells9102195. Cells. 2020. PMID: 33003357 Free PMC article. Review. - Conformational switches control early maturation of the eukaryotic small ribosomal subunit.
Hunziker M, Barandun J, Buzovetsky O, Steckler C, Molina H, Klinge S. Hunziker M, et al. Elife. 2019 Jun 17;8:e45185. doi: 10.7554/eLife.45185. Elife. 2019. PMID: 31206356 Free PMC article.
References
- Mol Gen Genet. 1977 Dec 30;158(2):117-22 - PubMed
- EMBO J. 1992 Apr;11(4):1531-42 - PubMed
- Proc Natl Acad Sci U S A. 1981 Apr;78(4):2258-62 - PubMed
- Cell. 1981 Nov;27(1 Pt 2):165-74 - PubMed
- Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases