An essential heparin-binding domain in the fibroblast growth factor receptor kinase - PubMed (original) (raw)
. 1993 Mar 26;259(5103):1918-21.
doi: 10.1126/science.8456318.
Affiliations
- PMID: 8456318
- DOI: 10.1126/science.8456318
An essential heparin-binding domain in the fibroblast growth factor receptor kinase
M Kan et al. Science. 1993.
Abstract
Heparin or heparin-like heparan sulfate proteoglycans are obligatory for activity of the heparin-binding fibroblast growth factor (FGF) family. Heparin interacts independently of FGF ligand with a specific sequence (K18K) in one of the immunoglobulin-like loops in the extracellular domain of the FGF receptor tyrosine kinase transmembrane glycoprotein. A synthetic peptide corresponding to K18K inhibited heparin and heparin-dependent FGF binding to the receptor. K18K and an antibody to K18K were antagonists of FGF-stimulated cell growth. Point mutations of lysine residues in the K18K sequence abrogated both heparin- and ligand-binding activities of the receptor kinase. The results indicate that the FGF receptor is a ternary complex of heparan sulfate proteoglycan, tyrosine kinase transmembrane glycoprotein, and ligand.
Similar articles
- Two hierarchies of FGF-2 signaling in heparin: mitogenic stimulation and high-affinity binding/receptor transphosphorylation.
Krufka A, Guimond S, Rapraeger AC. Krufka A, et al. Biochemistry. 1996 Aug 27;35(34):11131-41. doi: 10.1021/bi960125+. Biochemistry. 1996. PMID: 8780517 - Heparan sulfate fibroblast growth factor receptor complex: structure-function relationships.
McKeehan WL, Kan M. McKeehan WL, et al. Mol Reprod Dev. 1994 Sep;39(1):69-81; discusison 81-2. doi: 10.1002/mrd.1080390112. Mol Reprod Dev. 1994. PMID: 7999363 Review. - Common and specific determinants for fibroblast growth factors in the ectodomain of the receptor kinase complex.
Wang F, Lu W, McKeehan K, Mohamedali K, Gabriel JL, Kan M, McKeehan WL. Wang F, et al. Biochemistry. 1999 Jan 5;38(1):160-71. doi: 10.1021/bi981758m. Biochemistry. 1999. PMID: 9890894 - Distinct role of 2-O-, N-, and 6-O-sulfate groups of heparin in the formation of the ternary complex with basic fibroblast growth factor and soluble FGF receptor-1.
Rusnati M, Coltrini D, Caccia P, Dell'Era P, Zoppetti G, Oreste P, Valsasina B, Presta M. Rusnati M, et al. Biochem Biophys Res Commun. 1994 Aug 30;203(1):450-8. doi: 10.1006/bbrc.1994.2203. Biochem Biophys Res Commun. 1994. PMID: 8074689 - Tumor targeting through fibroblast growth factor receptors.
Lappi DA. Lappi DA. Semin Cancer Biol. 1995 Oct;6(5):279-88. doi: 10.1006/scbi.1995.0036. Semin Cancer Biol. 1995. PMID: 8562905 Review.
Cited by
- On Connective Tissue Mast Cells as Protectors of Life, Reproduction, and Progeny.
Norrby K. Norrby K. Int J Mol Sci. 2024 Apr 19;25(8):4499. doi: 10.3390/ijms25084499. Int J Mol Sci. 2024. PMID: 38674083 Free PMC article. Review. - Computer-aided engineering of stabilized fibroblast growth factor 21.
de La Bourdonnaye G, Ghazalova T, Fojtik P, Kutalkova K, Bednar D, Damborsky J, Rotrekl V, Stepankova V, Chaloupkova R. de La Bourdonnaye G, et al. Comput Struct Biotechnol J. 2024 Feb 7;23:942-951. doi: 10.1016/j.csbj.2024.02.001. eCollection 2024 Dec. Comput Struct Biotechnol J. 2024. PMID: 38379823 Free PMC article. - Glypican 4 mediates Wnt transport between germ layers via signaling filopodia.
Hu B, Rodriguez JJ, Kakkerla Balaraju A, Gao Y, Nguyen NT, Steen H, Suhaib S, Chen S, Lin F. Hu B, et al. J Cell Biol. 2021 Dec 6;220(12):e202009082. doi: 10.1083/jcb.202009082. Epub 2021 Sep 30. J Cell Biol. 2021. PMID: 34591076 Free PMC article. - Osteogenic and Angiogenic Properties of Heparin as a System for Delivery of Biomolecules for Bone Bioengineering: a Brief Critical Review.
Litvinova LS, Yurova KA, Khaziakhmatova OG, Khlusova MY, Malashchenko VV, Shunkin EO, Todosenko NM, Norkin IK, Ivanov PA, Khlusov IA. Litvinova LS, et al. Biochem Mosc Suppl B Biomed Chem. 2021;15(2):147-152. doi: 10.1134/S1990750821020050. Epub 2021 May 14. Biochem Mosc Suppl B Biomed Chem. 2021. PMID: 34007413 Free PMC article. - NMR Characterization of the Interactions Between Glycosaminoglycans and Proteins.
Bu C, Jin L. Bu C, et al. Front Mol Biosci. 2021 Mar 16;8:646808. doi: 10.3389/fmolb.2021.646808. eCollection 2021. Front Mol Biosci. 2021. PMID: 33796549 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical