Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism - PubMed (original) (raw)

Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism

P H Maxwell et al. Proc Natl Acad Sci U S A. 1993.

Abstract

Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is wide-spread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia.

PubMed Disclaimer

Similar articles

• Oxygen-regulated control elements in the phosphoglycerate kinase 1 and lactate dehydrogenase A genes: similarities with the erythropoietin 3' enhancer.
  Firth JD, Ebert BL, Pugh CW, Ratcliffe PJ. Firth JD, et al. Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6496-500. doi: 10.1073/pnas.91.14.6496. Proc Natl Acad Sci U S A. 1994. PMID: 8022811 Free PMC article.
• Functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.
  Pugh CW, Tan CC, Jones RW, Ratcliffe PJ. Pugh CW, et al. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10553-7. doi: 10.1073/pnas.88.23.10553. Proc Natl Acad Sci U S A. 1991. PMID: 1961720 Free PMC article.
• Diphenylene iodonium inhibits the induction of erythropoietin and other mammalian genes by hypoxia. Implications for the mechanism of oxygen sensing.
  Gleadle JM, Ebert BL, Ratcliffe PJ. Gleadle JM, et al. Eur J Biochem. 1995 Nov 15;234(1):92-9. doi: 10.1111/j.1432-1033.1995.092_c.x. Eur J Biochem. 1995. PMID: 8529674
• Regulation of expression of the erythropoietin gene.
  Maxwell P, Ratcliffe P. Maxwell P, et al. Curr Opin Hematol. 1998 May;5(3):166-70. doi: 10.1097/00062752-199805000-00003. Curr Opin Hematol. 1998. PMID: 9664154 Review.
• Hypoxia-inducible gene expression.
  Fandrey J. Fandrey J. Respir Physiol. 1995 Jul;101(1):1-10. doi: 10.1016/0034-5687(95)00013-4. Respir Physiol. 1995. PMID: 8525116 Review.

Cited by

• Determinants of ventilation and pulmonary artery pressure during early acclimatization to hypoxia in humans.
  Fatemian M, Herigstad M, Croft QP, Formenti F, Cardenas R, Wheeler C, Smith TG, Friedmannova M, Dorrington KL, Robbins PA. Fatemian M, et al. J Physiol. 2016 Mar 1;594(5):1197-213. doi: 10.1113/JP270061. Epub 2015 Jun 5. J Physiol. 2016. PMID: 25907672 Free PMC article.
• Reversion of lethality and growth defects in Fatiga oxygen-sensor mutant flies by loss of hypoxia-inducible factor-alpha/Sima.
  Centanin L, Ratcliffe PJ, Wappner P. Centanin L, et al. EMBO Rep. 2005 Nov;6(11):1070-5. doi: 10.1038/sj.embor.7400528. Epub 2005 Sep 23. EMBO Rep. 2005. PMID: 16179946 Free PMC article.
• The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes.
  Chavez JC, Baranova O, Lin J, Pichiule P. Chavez JC, et al. J Neurosci. 2006 Sep 13;26(37):9471-81. doi: 10.1523/JNEUROSCI.2838-06.2006. J Neurosci. 2006. PMID: 16971531 Free PMC article.
• Hypoxia signaling pathways in cancer metabolism: the importance of co-selecting interconnected physiological pathways.
  Masson N, Ratcliffe PJ. Masson N, et al. Cancer Metab. 2014 Feb 4;2(1):3. doi: 10.1186/2049-3002-2-3. Cancer Metab. 2014. PMID: 24491179 Free PMC article.
• Gene therapy for long-term expression of erythropoietin in rats.
  Osborne WR, Ramesh N, Lau S, Clowes MM, Dale DC, Clowes AW. Osborne WR, et al. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):8055-8. doi: 10.1073/pnas.92.17.8055. Proc Natl Acad Sci U S A. 1995. PMID: 7644537 Free PMC article.

References

1. 1. Nat New Biol. 1971 Nov 3;234(44):20-4 - PubMed
2. 1. Am J Physiol. 1992 Sep;263(3 Pt 2):F474-81 - PubMed
3. 1. J Cell Biol. 1974 Nov;63(2 Pt 1):414-9 - PubMed
4. 1. Science. 1983 Sep 23;221(4617):1283-5 - PubMed
5. 1. Mol Cell Biol. 1986 Mar;6(3):849-58 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database