Expression patterns of the cell adhesion molecule Nr-CAM during histogenesis of the chick nervous system - PubMed (original) (raw)
Expression patterns of the cell adhesion molecule Nr-CAM during histogenesis of the chick nervous system
L A Krushel et al. Neuroscience. 1993 Apr.
Abstract
Neuron-glia-related cell adhesion molecule (Nr-CAM) is a recently characterized cell adhesion molecule in the family of immunoglobulin-related molecules of which the neural cell adhesion molecule, N-CAM, is the prototype. Nr-CAM shares structural properties with another member of this family (neuron-glia CAM, Ng-CAM) and both molecules exhibit homophilic and heterophilic binding properties. To understand better the role of such molecules in development, we have examined the sites of synthesis and expression of Nr-CAM by means of in situ hybridization and immunohistochemistry. Both methods indicated that Nr-CAM is expressed only in the nervous system. The molecule was observed on neurons in both the peripheral and central nervous systems and on epithelial floor plate cells in the spinal cord, but it was absent in the germinal zones. The protein was present on perikarya, but was found preferentially on axonal tracts. As observed for messenger RNAs specifying other cell adhesion molecules, messenger RNA for Nr-CAM was localized in the perikarya. The temporal expression of Nr-CAM was correlated with various neural morphoregulatory events, including cell proliferation and migration, axonal outgrowth and myelination. The molecule was expressed during the onset of neurogenesis at embryonic day 3 in the floor plate epithelium, and then on postmitotic ventral horn motor neurons of the spinal cord. At later stages, it was expressed throughout the spinal cord but disappeared from the floor plate. In the cerebellum, Nr-CAM was found on granule and Purkinje neurons and afferent fibers. Both local and projection neurons in the optic tectum, as well as axonal pathways throughout the telencephalon, expressed Nr-CAM. In the peripheral nervous system, Nr-CAM was expressed strongly in sensory and autonomic ganglia and in the enteric nervous system. At the onset of myelination, there was a general decrease in staining for Nr-CAM protein in the central nervous system but not in the periphery. Comparison of the expression of Nr-CAM to that of the structurally related Ng-CAM showed considerable overlap in their distributions, although there were differences in the levels at which each CAM was observed in particular structures. For example, sympathetic ganglia stained more intensely for Nr-CAM protein than for Ng-CAM. This differential but co-distributed pattern is consistent with the idea that although similar cell adhesion molecules have independent binding specificities, they may have related functions that act synergistically in the development of the nervous system.
Similar articles
- Nr-CAM expression in the developing mouse nervous system: ventral midline structures, specific fiber tracts, and neuropilar regions.
Lustig M, Erskine L, Mason CA, Grumet M, Sakurai T. Lustig M, et al. J Comp Neurol. 2001 May 21;434(1):13-28. doi: 10.1002/cne.1161. J Comp Neurol. 2001. PMID: 11329126 - Differential distribution of cell adhesion molecules during histogenesis of the chick nervous system.
Daniloff JK, Chuong CM, Levi G, Edelman GM. Daniloff JK, et al. J Neurosci. 1986 Mar;6(3):739-58. doi: 10.1523/JNEUROSCI.06-03-00739.1986. J Neurosci. 1986. PMID: 2420947 Free PMC article. - Initial appearance and regional distribution of the neuron-glia cell adhesion molecule in the chick embryo.
Thiery JP, Delouvée A, Grumet M, Edelman GM. Thiery JP, et al. J Cell Biol. 1985 Feb;100(2):442-56. doi: 10.1083/jcb.100.2.442. J Cell Biol. 1985. PMID: 3881458 Free PMC article. - Nr-CAM: a cell adhesion molecule with ligand and receptor functions.
Grumet M. Grumet M. Cell Tissue Res. 1997 Nov;290(2):423-8. doi: 10.1007/s004410050949. Cell Tissue Res. 1997. PMID: 9321706 Review.
Cited by
- Dorsal commissural axon guidance in the developing spinal cord.
Alvarez S, Varadarajan SG, Butler SJ. Alvarez S, et al. Curr Top Dev Biol. 2021;142:197-231. doi: 10.1016/bs.ctdb.2020.10.009. Epub 2020 Nov 19. Curr Top Dev Biol. 2021. PMID: 33706918 Free PMC article. Review. - Xenopus laevis neuronal cell adhesion molecule (nrcam): plasticity of a CAM in the developing nervous system.
Lokapally A, Metikala S, Hollemann T. Lokapally A, et al. Dev Genes Evol. 2017 Jan;227(1):61-67. doi: 10.1007/s00427-016-0569-9. Epub 2016 Dec 10. Dev Genes Evol. 2017. PMID: 27942869 - Bilaterally symmetric populations of chicken dI1 (commissural) axons cross the floor plate independently of each other.
Phan KD, Butler SJ. Phan KD, et al. PLoS One. 2013 Apr 30;8(4):e62977. doi: 10.1371/journal.pone.0062977. Print 2013. PLoS One. 2013. PMID: 23646165 Free PMC article. - Overlapping functions of the cell adhesion molecules Nr-CAM and L1 in cerebellar granule cell development.
Sakurai T, Lustig M, Babiarz J, Furley AJ, Tait S, Brophy PJ, Brown SA, Brown LY, Mason CA, Grumet M. Sakurai T, et al. J Cell Biol. 2001 Sep 17;154(6):1259-73. doi: 10.1083/jcb.200104122. J Cell Biol. 2001. PMID: 11564762 Free PMC article. - A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.
Fitzli D, Stoeckli ET, Kunz S, Siribour K, Rader C, Kunz B, Kozlov SV, Buchstaller A, Lane RP, Suter DM, Dreyer WJ, Sonderegger P. Fitzli D, et al. J Cell Biol. 2000 May 15;149(4):951-68. doi: 10.1083/jcb.149.4.951. J Cell Biol. 2000. PMID: 10811834 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous