Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control - PubMed (original) (raw)
Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control
T Toda et al. EMBO J. 1993 May.
Abstract
Two novel protein kinase C (PKC)-like genes, pck1+ and pck2+ were isolated from fission yeast by PCR. Both contain common domains of PKC-related molecules, but lack a putative Ca(2+)-binding domain so that they may belong to the nPKC group. Gene disruption of pck1+ and pck2+ establishes that they share an overlapping essential function for cell viability. Cells of a single pck2 deletion display severe defects in cell shape; they are irregular and sometimes pear-like instead of cylindrical. In contrast, the induced overexpression of pck2+ is lethal, producing multiseptated and branched cells. These results suggest that fission yeast PKC-like genes are involved in the polarity of cell growth control. We show that pck2 is allelic to sts6, a locus we have previously identified by its supersensitivity to staurosporine, a potent protein kinase inhibitor [Toda et al. (1991) Genes Dev., 5, 60-73]. In addition, the lethal overexpression of pck2+ can be suppressed by staurosporine, indicating that fission yeast pck1 and pck2 are molecular targets of this inhibitor.
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References
- Cell. 1984 May;37(1):67-75 - PubMed
- Annu Rev Biochem. 1989;58:453-508 - PubMed
- Nature. 1988 Aug 25;334(6184):661-5 - PubMed
- Cell. 1988 Jun 3;53(5):731-41 - PubMed
- Nature. 1987 May 7-13;327(6117):31-5 - PubMed
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