Copy number of the 16S rRNA gene in Rickettsia prowazekii - PubMed (original) (raw)
Copy number of the 16S rRNA gene in Rickettsia prowazekii
H Pang et al. J Bacteriol. 1993 Jun.
Abstract
The obligate intracellular parasite, Rickettsia prowazekii, is a slowly growing bacterium with a doubling time of 8 to 12 h. The copy number of the 16S rRNA gene in the rickettsial chromosome was determined to be one. Genomic DNA from R. prowazekii was digested either by a variety of restriction enzymes known not to cut at any site in the rickettsial 16S rRNA gene or by a combination of these noncutting enzymes and SmaI, which cuts the gene only once. Only one DNA fragment in these digests hybridized to a biotinylated probe containing a portion of the rickettsial 16S rRNA gene. Moreover, the density of the rickettsial 16S rRNA gene fragment after hybridization was equal to the density of each of the seven 16S rRNA gene fragments in Escherichia coli.
Similar articles
- Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii.
Pang H, Winkler HH. Pang H, et al. J Bacteriol. 1996 Mar;178(6):1750-5. doi: 10.1128/jb.178.6.1750-1755.1996. J Bacteriol. 1996. PMID: 8626305 Free PMC article. - Unusual organization of the rRNA genes in Rickettsia prowazekii.
Andersson SG, Zomorodipour A, Winkler HH, Kurland CG. Andersson SG, et al. J Bacteriol. 1995 Jul;177(14):4171-5. doi: 10.1128/jb.177.14.4171-4175.1995. J Bacteriol. 1995. PMID: 7608097 Free PMC article. - [Homology between the ribosomal and RNA-polymerase genes of E. coli and vaccine strain E of Rickettsia prowazekii].
Artem'ev MI, Balaeva NM. Artem'ev MI, et al. Mol Gen Mikrobiol Virusol. 1987 May;(5):34-6. Mol Gen Mikrobiol Virusol. 1987. PMID: 3039361 Russian. - Progress in rickettsial genome analysis from pioneering of Rickettsia prowazekii to the recent Rickettsia typhi.
Walker DH, Yu XJ. Walker DH, et al. Ann N Y Acad Sci. 2005 Dec;1063:13-25. doi: 10.1196/annals.1355.003. Ann N Y Acad Sci. 2005. PMID: 16481486 Review. - Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches.
Tucker AM, Pannell LK, Wood DO. Tucker AM, et al. Ann N Y Acad Sci. 2005 Dec;1063:35-46. doi: 10.1196/annals.1355.005. Ann N Y Acad Sci. 2005. PMID: 16481488 Review.
Cited by
- Origin and evolution of the mitochondrial proteome.
Kurland CG, Andersson SG. Kurland CG, et al. Microbiol Mol Biol Rev. 2000 Dec;64(4):786-820. doi: 10.1128/MMBR.64.4.786-820.2000. Microbiol Mol Biol Rev. 2000. PMID: 11104819 Free PMC article. Review. - Acquisition and transmission of the agent of human granulocytic ehrlichiosis by Ixodes scapularis ticks.
Hodzic E, Fish D, Maretzki CM, De Silva AM, Feng S, Barthold SW. Hodzic E, et al. J Clin Microbiol. 1998 Dec;36(12):3574-8. doi: 10.1128/JCM.36.12.3574-3578.1998. J Clin Microbiol. 1998. PMID: 9817875 Free PMC article. - Synthesis of functional eukaryotic ribosomal RNAs in trans: development of a novel in vivo rDNA system for dissecting ribosome biogenesis.
Liang WQ, Fournier MJ. Liang WQ, et al. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2864-8. doi: 10.1073/pnas.94.7.2864. Proc Natl Acad Sci U S A. 1997. PMID: 9096312 Free PMC article. - A chimeric disposition of the elongation factor genes in Rickettsia prowazekii.
Syvänen AC, Amiri H, Jamal A, Andersson SG, Kurland CG. Syvänen AC, et al. J Bacteriol. 1996 Nov;178(21):6192-9. doi: 10.1128/jb.178.21.6192-6199.1996. J Bacteriol. 1996. PMID: 8892818 Free PMC article.
References
- Infect Immun. 1981 Nov;34(2):596-604 - PubMed
- J Bacteriol. 1984 Apr;158(1):376-8 - PubMed
- Cell. 1986 Jan 17;44(1):197-205 - PubMed
- Biochem Biophys Res Commun. 1986 May 14;136(3):1136-41 - PubMed
- J Bacteriol. 1989 Jan;171(1):70-3 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources