Microsatellite instability, mismatch repair deficiency, and genetic defects in human cancer cell lines - PubMed (original) (raw)
. 1995 Dec 15;55(24):6063-70.
Affiliations
- PMID: 8521394
Microsatellite instability, mismatch repair deficiency, and genetic defects in human cancer cell lines
J C Boyer et al. Cancer Res. 1995.
Abstract
The instability of short repetitive sequences in tumor DNA can result from defective repair of replication errors due to mutations in any of several genes required for mismatch repair. Understanding this repair pathway and how defects lead to cancer is being facilitated by genetic and biochemical studies of tumor cell lines. In the present study, we describe the mismatch repair status of extracts of 22 tumor cell lines derived from several tissue types. Ten were found to be defective in strand-specific mismatch repair, including cell lines from tumors of the colon, ovary, endometrium, and prostate. The repair defects were independent of whether the signal for strand specificity, a nick, was 5' or 3' to the mismatch. All 10 defective cell lines exhibited microsatellite instability. Repair activity was restored to 9 of these 10 extracts by adding a second defective extract made from cell lines having known mutations in either the hMSH2 or hMLH1 genes. Subsequent analyses revealed mutations in hMSH2 (4 lines) and hMLH1 (5 lines) that could explain the observed microsatellite instability and repair defects. Overall, this study strengthens the correlation between microsatellite instability and defective mismatch repair and the suggestion that diminuition in mismatch repair activity is a step in carcinogenesis common to several types of cancer. It also provides an extensive panel of repair-proficient and repair-deficient cell lines for future studies of mismatch repair.
Similar articles
- Human colon cancer cells surviving high doses of cisplatin or oxaliplatin in vitro are not defective in DNA mismatch repair proteins.
Sergent C, Franco N, Chapusot C, Lizard-Nacol S, Isambert N, Correia M, Chauffert B. Sergent C, et al. Cancer Chemother Pharmacol. 2002 Jun;49(6):445-52. doi: 10.1007/s00280-002-0450-6. Epub 2002 Apr 20. Cancer Chemother Pharmacol. 2002. PMID: 12107548 - Transient mismatch repair gene transfection for functional analysis of genetic hMLH1 and hMSH2 variants.
Brieger A, Trojan J, Raedle J, Plotz G, Zeuzem S. Brieger A, et al. Gut. 2002 Nov;51(5):677-84. doi: 10.1136/gut.51.5.677. Gut. 2002. PMID: 12377806 Free PMC article. - DNA mismatch repair enzyme activity and gene expression in prostate cancer.
Yeh CC, Lee C, Dahiya R. Yeh CC, et al. Biochem Biophys Res Commun. 2001 Jul 13;285(2):409-13. doi: 10.1006/bbrc.2001.5187. Biochem Biophys Res Commun. 2001. PMID: 11444857 - Microsatellite instability in human solid tumors.
Lothe RA. Lothe RA. Mol Med Today. 1997 Feb;3(2):61-8. doi: 10.1016/S1357-4310(96)10055-1. Mol Med Today. 1997. PMID: 9060003 Review. - Differences and evolution of the methods for the assessment of microsatellite instability.
Laghi L, Bianchi P, Malesci A. Laghi L, et al. Oncogene. 2008 Oct 23;27(49):6313-21. doi: 10.1038/onc.2008.217. Epub 2008 Aug 4. Oncogene. 2008. PMID: 18679418 Review.
Cited by
- Death receptors 4/5 mediate tumour sensitivity to natural killer cell-mediated cytotoxicity in mismatch repair deficient colorectal cancer.
Yang L, Yi J, He W, Kong P, Xie Q, Jin Y, Xiong Z, Xia L. Yang L, et al. Br J Cancer. 2024 Jul;131(2):334-346. doi: 10.1038/s41416-024-02673-z. Epub 2024 May 25. Br J Cancer. 2024. PMID: 38796599 - Activation of recombinational repair in Ewing sarcoma cells carrying EWS-FLI1 fusion gene by chromosome translocation.
Tanaka K, Suzuki K, Miyashita K, Wakasa K, Kawano M, Nakatsu Y, Tsumura H, Yoshida MA, Oda S. Tanaka K, et al. Sci Rep. 2022 Aug 30;12(1):14764. doi: 10.1038/s41598-022-19164-x. Sci Rep. 2022. PMID: 36042341 Free PMC article. - Insights on Microsatellite Characteristics, Evolution, and Function From the Social Amoeba Dictyostelium discoideum.
Williams FN, Scaglione KM. Williams FN, et al. Front Neurosci. 2022 Jun 13;16:886837. doi: 10.3389/fnins.2022.886837. eCollection 2022. Front Neurosci. 2022. PMID: 35769695 Free PMC article. Review. - In-silico and in-vitro studies on the efficacy of mangiferin against colorectal cancer.
Samadarsi R, Augustin L, Kumar C, Dutta D. Samadarsi R, et al. BMC Chem. 2022 Jun 7;16(1):42. doi: 10.1186/s13065-022-00835-9. BMC Chem. 2022. PMID: 35672858 Free PMC article. - Acquired resistance to irradiation or docetaxel is not associated with cross-resistance to cisplatin in prostate cancer cell lines.
Donix L, Erb HHH, Peitzsch C, Dubrovska A, Pfeifer M, Thomas C, Fuessel S, Erdmann K. Donix L, et al. J Cancer Res Clin Oncol. 2022 Jun;148(6):1313-1324. doi: 10.1007/s00432-022-03914-5. Epub 2022 Jan 12. J Cancer Res Clin Oncol. 2022. PMID: 35020044 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources