Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix - PubMed (original) (raw)
Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix
C Wu et al. Cell. 1995.
Free article
Abstract
Fibronectin (Fn) matrices are vital to vertebrate development and wound healing and modulate tumorigenesis. We used a recombinant Fn-binding integrin alpha IIb beta3, to define rules for integrin-initiated Fn matrix formation. We report the following. First, multiple Fn-binding integrins can support matrix assembly; their activation state controls fibrillogenesis. Second, Fn binding to cells expressing an activated integrin is necessary but not sufficient for matrix assembly. Additional "postoccupancy" events involving the integrin beta, but not the alpha subunit, cytoplasmic domain are needed. Third, these postoccupancy events require an intact actin cytoskeleton. We propose a model for integrin involvement in Fn fibrillogenesis that reconciles previous paradoxes and suggests novel approaches to the therapeutic control of Fn matrix assembly.
Similar articles
- Coordinated regulation of fibronectin fibril assembly and actin stress fiber formation.
Sechler JL, Schwarzbauer JE. Sechler JL, et al. Cell Adhes Commun. 1997 Mar;4(6):413-24. doi: 10.3109/15419069709004458. Cell Adhes Commun. 1997. PMID: 9177903 - Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis.
Pankov R, Cukierman E, Katz BZ, Matsumoto K, Lin DC, Lin S, Hahn C, Yamada KM. Pankov R, et al. J Cell Biol. 2000 Mar 6;148(5):1075-90. doi: 10.1083/jcb.148.5.1075. J Cell Biol. 2000. PMID: 10704455 Free PMC article. - The role of integrin binding sites in fibronectin matrix assembly in vivo.
Leiss M, Beckmann K, Girós A, Costell M, Fässler R. Leiss M, et al. Curr Opin Cell Biol. 2008 Oct;20(5):502-7. doi: 10.1016/j.ceb.2008.06.001. Epub 2008 Jul 21. Curr Opin Cell Biol. 2008. PMID: 18586094 Review. - Fibronectin fibrillogenesis, a cell-mediated matrix assembly process.
Mao Y, Schwarzbauer JE. Mao Y, et al. Matrix Biol. 2005 Sep;24(6):389-99. doi: 10.1016/j.matbio.2005.06.008. Matrix Biol. 2005. PMID: 16061370 Review.
Cited by
- The distribution and regulation of integrin-linked kinase in normal and diabetic kidneys.
Guo L, Sanders PW, Woods A, Wu C. Guo L, et al. Am J Pathol. 2001 Nov;159(5):1735-42. doi: 10.1016/S0002-9440(10)63020-9. Am J Pathol. 2001. PMID: 11696434 Free PMC article. - R-Ras regulates migration through an interaction with filamin A in melanoma cells.
Gawecka JE, Griffiths GS, Ek-Rylander B, Ramos JW, Matter ML. Gawecka JE, et al. PLoS One. 2010 Jun 23;5(6):e11269. doi: 10.1371/journal.pone.0011269. PLoS One. 2010. PMID: 20585650 Free PMC article. - An essential role for thymic mesenchyme in early T cell development.
Suniara RK, Jenkinson EJ, Owen JJ. Suniara RK, et al. J Exp Med. 2000 Mar 20;191(6):1051-6. doi: 10.1084/jem.191.6.1051. J Exp Med. 2000. PMID: 10727466 Free PMC article. - Fibulin-5, an integrin-binding matricellular protein: its function in development and disease.
Yanagisawa H, Schluterman MK, Brekken RA. Yanagisawa H, et al. J Cell Commun Signal. 2009 Dec;3(3-4):337-47. doi: 10.1007/s12079-009-0065-3. Epub 2009 Oct 2. J Cell Commun Signal. 2009. PMID: 19798595 Free PMC article. - Minimally modified low-density lipoprotein induces monocyte adhesion to endothelial connecting segment-1 by activating beta1 integrin.
Shih PT, Elices MJ, Fang ZT, Ugarova TP, Strahl D, Territo MC, Frank JS, Kovach NL, Cabanas C, Berliner JA, Vora DK. Shih PT, et al. J Clin Invest. 1999 Mar;103(5):613-25. doi: 10.1172/JCI5710. J Clin Invest. 1999. PMID: 10074478 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous