Purification and properties of an ATP-dependent nucleosome remodeling factor - PubMed (original) (raw)
Purification and properties of an ATP-dependent nucleosome remodeling factor
T Tsukiyama et al. Cell. 1995.
Free article
Abstract
We report the purification of an ATP-dependent nucleosome remodeling factor (NURF) from Drosophila embryo extracts. NURF is composed of at least four polypeptides that act in concert with the GAGA transcription factor to alter chromatin structure at the hsp70 promoter. The energy requirement is attributed to an ATPase activity that is stimulated by nucleosomes but not by free DNA or histones, suggesting that NURF acts directly on a nucleosome to perturb its structure. This finding and the physical properties of NURF contrast sharply with the multisubunit SWI2/SNF2 complex, which has also been shown to alter nucleosomes in an ATP-dependent manner. The results suggest that two distinct systems may be involved in remodeling chromatin for transcription.
Similar articles
- ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF.
Hamiche A, Sandaltzopoulos R, Gdula DA, Wu C. Hamiche A, et al. Cell. 1999 Jun 25;97(7):833-42. doi: 10.1016/s0092-8674(00)80796-5. Cell. 1999. PMID: 10399912 - ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.
Tsukiyama T, Becker PB, Wu C. Tsukiyama T, et al. Nature. 1994 Feb 10;367(6463):525-32. doi: 10.1038/367525a0. Nature. 1994. PMID: 8107823 - Role of nucleosome remodeling factor NURF in transcriptional activation of chromatin.
Mizuguchi G, Tsukiyama T, Wisniewski J, Wu C. Mizuguchi G, et al. Mol Cell. 1997 Dec;1(1):141-50. doi: 10.1016/s1097-2765(00)80015-5. Mol Cell. 1997. PMID: 9659911 - What does 'chromatin remodeling' mean?
Aalfs JD, Kingston RE. Aalfs JD, et al. Trends Biochem Sci. 2000 Nov;25(11):548-55. doi: 10.1016/s0968-0004(00)01689-3. Trends Biochem Sci. 2000. PMID: 11084367 Review. - ATP-dependent remodeling of chromatin.
Wu C, Tsukiyama T, Gdula D, Georgel P, Martínez-Balbás M, Mizuguchi G, Ossipow V, Sandaltzopoulos R, Wang HM. Wu C, et al. Cold Spring Harb Symp Quant Biol. 1998;63:525-34. doi: 10.1101/sqb.1998.63.525. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384317 Review. No abstract available.
Cited by
- ATP-dependent chromatin remodeling by the Cockayne syndrome B DNA repair-transcription-coupling factor.
Citterio E, Van Den Boom V, Schnitzler G, Kanaar R, Bonte E, Kingston RE, Hoeijmakers JH, Vermeulen W. Citterio E, et al. Mol Cell Biol. 2000 Oct;20(20):7643-53. doi: 10.1128/MCB.20.20.7643-7653.2000. Mol Cell Biol. 2000. PMID: 11003660 Free PMC article. - Roles of histone acetylation modification in basal and inducible expression of hsp26 gene in D. melanogaster.
Zhao Y, Lu J, Sun H, Chen X, Huang B. Zhao Y, et al. Mol Cell Biochem. 2007 Dec;306(1-2):1-8. doi: 10.1007/s11010-007-9547-x. Epub 2007 Jul 10. Mol Cell Biochem. 2007. PMID: 17619947 - Two distinct mechanisms of chromatin interaction by the Isw2 chromatin remodeling complex in vivo.
Fazzio TG, Gelbart ME, Tsukiyama T. Fazzio TG, et al. Mol Cell Biol. 2005 Nov;25(21):9165-74. doi: 10.1128/MCB.25.21.9165-9174.2005. Mol Cell Biol. 2005. PMID: 16227570 Free PMC article. - Nucleolin is required for RNA polymerase I transcription in vivo.
Rickards B, Flint SJ, Cole MD, LeRoy G. Rickards B, et al. Mol Cell Biol. 2007 Feb;27(3):937-48. doi: 10.1128/MCB.01584-06. Epub 2006 Nov 27. Mol Cell Biol. 2007. PMID: 17130237 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases