Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein - PubMed (original) (raw)

Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein

J B Yoon et al. Mol Cell Biol. 1996 Jan.

Abstract

The proximal sequence element (PSE)-binding transcription factor (PTF) specifically recognizes the PSEs of both RNA polymerase II- and RNA polymerase III-transcribed small nuclear RNA (snRNA) genes. We previously have shown that PTF purified from human HeLa cells is a multisubunit complex of four polypeptides designated PTF alpha, -beta, -gamma, and -delta. We now report the isolation and expression of cDNAs encoding PTF gamma and PTF delta, as well as functional studies with cognate antibodies that recognize the native PTF complex in HeLa extracts. Immunoprecipitation studies confirm that the four PTF subunits originally found to copurify during conventional chromatography indeed form a tightly associated complex; they further show that the PTF so defined, including the gamma and delta subunits specifically, is essential for transcription of both class II and class III snRNA genes. Immunoprecipitation assays also show a weak substoichiometric association of the TATA-binding protein (TBP) with PTF, consistent with the previous report of a PTF-related complex (SNAPc) containing substoichiometric levels of TBP and a component (SNAPc43) identical in sequence to the PTF gamma reported here. Glutathione S-transferase pulldown assays further indicate relatively strong direct interactions of both recombinant PTF gamma and PTF delta with TBP, consistent either with the natural association of TBP with PTF in a semistable TBP-TBP-associated factor complex or with possible functional interactions between PSE-bound PTF and TATA-bound TBP during promoter activation. In addition, we show that in extracts depleted of TBP and TBP-associated factors, transcription from the U1 promoter is restored by recombinant TBP but not by TFIID or TFIIIB, indicating that transcription of class II snRNA genes requires a TBP complex different from the one used for mRNA-encoding genes.

PubMed Disclaimer

Cited by

The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.
Kuhlman TC, Cho H, Reinberg D, Hernandez N. Kuhlman TC, et al. Mol Cell Biol. 1999 Mar;19(3):2130-41. doi: 10.1128/MCB.19.3.2130. Mol Cell Biol. 1999. PMID: 10022900 Free PMC article.
Human snRNA genes use polyadenylation factors to promote efficient transcription termination.
O'Reilly D, Kuznetsova OV, Laitem C, Zaborowska J, Dienstbier M, Murphy S. O'Reilly D, et al. Nucleic Acids Res. 2014 Jan;42(1):264-75. doi: 10.1093/nar/gkt892. Epub 2013 Oct 4. Nucleic Acids Res. 2014. PMID: 24097444 Free PMC article.
Cloning and characterization of SNAP50, a subunit of the snRNA-activating protein complex SNAPc.
Henry RW, Ma B, Sadowski CL, Kobayashi R, Hernandez N. Henry RW, et al. EMBO J. 1996 Dec 16;15(24):7129-36. EMBO J. 1996. PMID: 9003788 Free PMC article.
The SNAP45 subunit of the small nuclear RNA (snRNA) activating protein complex is required for RNA polymerase II and III snRNA gene transcription and interacts with the TATA box binding protein.
Sadowski CL, Henry RW, Kobayashi R, Hernandez N. Sadowski CL, et al. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4289-93. doi: 10.1073/pnas.93.9.4289. Proc Natl Acad Sci U S A. 1996. PMID: 8633057 Free PMC article.
The retinoblastoma tumor suppressor protein targets distinct general transcription factors to regulate RNA polymerase III gene expression.
Hirsch HA, Gu L, Henry RW. Hirsch HA, et al. Mol Cell Biol. 2000 Dec;20(24):9182-91. doi: 10.1128/MCB.20.24.9182-9191.2000. Mol Cell Biol. 2000. PMID: 11094070 Free PMC article.

References

1. J Biol Chem. 1990 Oct 15;265(29):17911-20 - PubMed
1. Biochim Biophys Acta. 1991 Jan 17;1088(1):1-9 - PubMed
1. Nucleic Acids Res. 1991 Feb 11;19(3):435-41 - PubMed
1. EMBO J. 1991 Jul;10(7):1853-62 - PubMed
1. Cell. 1991 Aug 9;66(3):563-76 - PubMed

Cloning of two proximal sequence element-binding transcription factor subunits (gamma and delta) that are required for transcription of small nuclear RNA genes by RNA polymerases II and III and interact with the TATA-binding protein - PubMed (original) (raw)