The ETS-domain transcription factors Elk-1 and SAP-1 exhibit differential DNA binding specificities - PubMed (original) (raw)

Comparative Study

. 1995 Nov 25;23(22):4698-706.

doi: 10.1093/nar/23.22.4698.

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Free PMC article

Comparative Study

The ETS-domain transcription factors Elk-1 and SAP-1 exhibit differential DNA binding specificities

P Shore et al. Nucleic Acids Res. 1995.

Free PMC article

Abstract

The ETS DNA-binding domain is conserved amongst many eukaryotic transcription factors. ETS-domains bind differentially to specific DNA sites containing a central GGA trinucleotide motif. The nucleotides flanking this motif define the binding specificity of individual proteins. In this study we have investigated binding specificity of the ETS-domains from two members of the ternary complex factor (TCF) subfamily, Elk-1 and SAP-1. The ETS DNA-binding domains of Elk-1 (Elk-93) and SAP-1 (SAP-92) select similar sites from random pools of double stranded oligonucleotides based on the consensus sequence ACCGGAAGTR. However, SAP-92 shows a more relaxed binding site selectivity and binds efficiently to a greater spectrum of sites than does Elk-93. This more relaxed DNA binding site selectivity is most pronounced in nucleotides located on the 3' side of the GGA motif. This differential DNA-binding specificity is also exhibited by longer TCF derivatives and, indeed by the full-length proteins. Our results suggest that the range of potential in vivo target sites for SAP-1 is likely to be greater than for Elk-1. We discuss our results in relation to other similar studies carried out with more divergent ETS-domains.

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