Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor - PubMed (original) (raw)

Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

R Calligaris et al. Proc Natl Acad Sci U S A. 1995.

Abstract

GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

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