Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes - PubMed (original) (raw)

Comparative Study

Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes

W C Au et al. Proc Natl Acad Sci U S A. 1995.

Abstract

A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. We report the identification of a member of the IRF family which we have named IRF-3. The IRF-3 gene is present in a single copy in human genomic DNA. It is expressed constitutively in a variety of tissues and no increase in the relative steady-state levels of IRF-3 mRNA was observed in virus-infected or IFN-treated cells. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. The murine IFNA4 promoter, which can be induced by IRF-1 or viral infection, is not induced by IRF-3. Expression of IRF-3 as a Gal4 fusion protein does not activate expression of a chloramphenicol acetyltransferase reporter gene containing repeats of the Gal4 binding sites, indicating that this protein does not contain the transcription transactivation domain. The high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family. Identification of this ISRE-binding protein may help us to understand the specificity in the various Stat pathways.

PubMed Disclaimer

Cited by

Herpes Simplex Virus Type 2 Blocks IFN-β Production through the Viral UL24 N-Terminal Domain-Mediated Inhibition of IRF-3 Phosphorylation.
Zhang B, Li Y, Yang P, He S, Li W, Li M, Hu Q, Zhang M. Zhang B, et al. Viruses. 2024 Oct 11;16(10):1601. doi: 10.3390/v16101601. Viruses. 2024. PMID: 39459934 Free PMC article.
ISG15 expression in response to double-stranded RNA or LPS in cultured Fetal bovine lung (FBL) cells.
Liu C, Chang R, Yao X, Qiao WT, Geng YQ. Liu C, et al. Vet Res Commun. 2009 Oct;33(7):723-33. doi: 10.1007/s11259-009-9221-8. Epub 2009 Apr 15. Vet Res Commun. 2009. PMID: 19367472
Inhibition of type I interferon production via suppressing IKK-gamma expression: a new strategy for counteracting host antiviral defense by influenza A viruses?
Wang Y, Zhou J, Ruan C, Du Y. Wang Y, et al. J Proteome Res. 2012 Jan 1;11(1):217-23. doi: 10.1021/pr200894t. Epub 2011 Nov 29. J Proteome Res. 2012. PMID: 22054014 Free PMC article.
The interferon-inducible murine p48 (ISGF3gamma) gene is regulated by protooncogene c-myc.
Weihua X, Lindner DJ, Kalvakolanu DV. Weihua X, et al. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7227-32. doi: 10.1073/pnas.94.14.7227. Proc Natl Acad Sci U S A. 1997. PMID: 9207073 Free PMC article.

References

1. Mol Cell Biol. 1995 May;15(5):2558-69 - PubMed
1. Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3829-33 - PubMed
1. Genes Dev. 1995 Jun 1;9(11):1377-87 - PubMed
1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 - PubMed
1. Nature. 1989 Jan 19;337(6204):270-2 - PubMed

Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes - PubMed (original) (raw)