Engineering an intracellular pathway for major histocompatibility complex class II presentation of antigens - PubMed (original) (raw)

Engineering an intracellular pathway for major histocompatibility complex class II presentation of antigens

T C Wu et al. Proc Natl Acad Sci U S A. 1995.

Abstract

The presentation of antigenic peptides by major histocompatibility complex (MHC) class II molecules to CD4+ T cells is critical to the function of the immune system. In this study, we have utilized the sorting signal of the lysosomal-associated membrane protein LAMP-1 to target a model antigen, human papillomavirus 16 E7 (HPV-16 E7), into the endosomal and lysosomal compartments. The LAMP-1 sorting signal reroutes the antigen into the MHC class II processing pathway, resulting in enhanced presentation to CD4+ cells in vitro. In vivo immunization experiments in mice demonstrated that vaccinia containing the chimeric E7/LAMP-1 gene generated greater E7-specific lymphoproliferative activity, antibody titers, and cytotoxic T-lymphocyte activities than vaccinia containing the wild-type HPV-16 E7 gene. These results suggest that specific targeting of an antigen to the endosomal and lysosomal compartments enhances MHC class II presentation and vaccine potency.

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References

    1. J Immunol. 1992 Jul 15;149(2):429-37 - PubMed
    1. Virology. 1989 May;170(1):311-5 - PubMed
    1. Trends Neurosci. 1989 Oct;12(10):400-6 - PubMed
    1. Curr Opin Immunol. 1994 Oct;6(5):746-54 - PubMed
    1. J Cell Biol. 1985 Jul;101(1):85-95 - PubMed

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