A protein linkage map of Escherichia coli bacteriophage T7 - PubMed (original) (raw)

A protein linkage map of Escherichia coli bacteriophage T7

P L Bartel et al. Nat Genet. 1996 Jan.

Abstract

Genome sequencing projects are predicting large numbers of novel proteins, whose interactions with other proteins must mediate the function of cellular processes. To analyse these networks, we used the yeast two-hybrid system on a genome-wide scale to identify 25 interactions among the proteins of Escherichia coli bacteriophage T7. Among these is a set of six interactions connecting proteins that function in DNA replication and DNA packaging. Remarkably, two genes, arranged such that one entirely overlaps the other and uses a different reading frame, encode interacting proteins. Several of the interactions reflect intramolecular associations of different domains of the same polypeptide, suggesting that the two-hybrid assay may be useful in the analysis of protein folding. This global approach to protein-protein interactions may be applicable to the analysis of more complex genomes whose sequences are becoming available.

PubMed Disclaimer

Similar articles

• Toward a functional analysis of the yeast genome through exhaustive two-hybrid screens.
  Fromont-Racine M, Rain JC, Legrain P. Fromont-Racine M, et al. Nat Genet. 1997 Jul;16(3):277-82. doi: 10.1038/ng0797-277. Nat Genet. 1997. PMID: 9207794
• Genetic selection for and molecular dynamic modeling of a protein transmembrane domain multimerization motif from a random Escherichia coli genomic library.
  Leeds JA, Boyd D, Huber DR, Sonoda GK, Luu HT, Engelman DM, Beckwith J. Leeds JA, et al. J Mol Biol. 2001 Oct 12;313(1):181-95. doi: 10.1006/jmbi.2001.5007. J Mol Biol. 2001. PMID: 11601855
• Co-expression of gene 31 and 23 products of bacteriophage T4.
  Kurochkina LP, Mesyanzhinov VV. Kurochkina LP, et al. Biochemistry (Mosc). 1999 Apr;64(4):379-83. Biochemistry (Mosc). 1999. PMID: 10231589
• Gp2.5, the multifunctional bacteriophage T7 single-stranded DNA binding protein.
  Hernandez AJ, Richardson CC. Hernandez AJ, et al. Semin Cell Dev Biol. 2019 Feb;86:92-101. doi: 10.1016/j.semcdb.2018.03.018. Epub 2018 Mar 28. Semin Cell Dev Biol. 2019. PMID: 29588157 Free PMC article. Review.
• Genome function--a virus-world view.
  Yin J. Yin J. Adv Exp Med Biol. 2004;547:31-46. doi: 10.1007/978-1-4419-8861-4_4. Adv Exp Med Biol. 2004. PMID: 15230091 Review.

Cited by

• Unraveling Protein Interactions between the Temperate Virus Bam35 and Its Bacillus Host Using an Integrative Yeast Two Hybrid-High Throughput Sequencing Approach.
  Lechuga A, Lood C, Berjón-Otero M, Del Prado A, Wagemans J, van Noort V, Lavigne R, Salas M, Redrejo-Rodríguez M. Lechuga A, et al. Int J Mol Sci. 2021 Oct 14;22(20):11105. doi: 10.3390/ijms222011105. Int J Mol Sci. 2021. PMID: 34681765 Free PMC article.
• Characterization of Two New Shiga Toxin-Producing Escherichia coli O103-Infecting Phages Isolated from an Organic Farm.
  Zhang Y, Liao YT, Salvador A, Lavenburg VM, Wu VCH. Zhang Y, et al. Microorganisms. 2021 Jul 17;9(7):1527. doi: 10.3390/microorganisms9071527. Microorganisms. 2021. PMID: 34361962 Free PMC article.
• Compartmentalization-aided interaction screening reveals extensive high-order complexes within the SARS-CoV-2 proteome.
  Xu W, Pei G, Liu H, Ju X, Wang J, Ding Q, Li P. Xu W, et al. Cell Rep. 2021 Aug 3;36(5):109482. doi: 10.1016/j.celrep.2021.109482. Epub 2021 Jul 17. Cell Rep. 2021. PMID: 34297909 Free PMC article.
• Programmable design of orthogonal protein heterodimers.
  Chen Z, Boyken SE, Jia M, Busch F, Flores-Solis D, Bick MJ, Lu P, VanAernum ZL, Sahasrabuddhe A, Langan RA, Bermeo S, Brunette TJ, Mulligan VK, Carter LP, DiMaio F, Sgourakis NG, Wysocki VH, Baker D. Chen Z, et al. Nature. 2019 Jan;565(7737):106-111. doi: 10.1038/s41586-018-0802-y. Epub 2018 Dec 19. Nature. 2019. PMID: 30568301 Free PMC article.
• Enzymes and Mechanisms Employed by Tailed Bacteriophages to Breach the Bacterial Cell Barriers.
  Fernandes S, São-José C. Fernandes S, et al. Viruses. 2018 Jul 27;10(8):396. doi: 10.3390/v10080396. Viruses. 2018. PMID: 30060520 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • Gene Ontology