Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant - PubMed (original) (raw)

Comparative Study

Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant

N A Machin et al. Mol Biol Cell. 1995 Sep.

Free PMC article

Abstract

To better understand the dynamic regulation of microtubule structures in yeast, we studied a conditional-lethal beta-tubulin mutation tub2-150. This mutation is unique among the hundreds of tubulin mutations isolated in Saccharomyces cerevisiae in that it appears to cause an increase in the stability of microtubules. We report here that this allele is a mutation of threonine 238 to alanine, and that tub2-150 prevents the spindle from elongating during anaphase, suggesting a nuclear microtubule defect. To identify regulators of microtubule stability and/or anaphase, yeast genes were selected that, when overexpressed, could suppress the tub2-150 temperature-sensitive phenotype. One of these genes, JSN1, encodes a protein of 125 kDa that has limited similarity to a number of proteins of unknown function. Overexpression of the JSN1 gene in a TUB2 strain causes that strain to become more sensitive to benomyl, a microtubule-destabilizing drug. Of a representative group of microtubule mutants, only one other mutation, tub2-404, could be suppressed by JSN1 overexpression, showing that JSN1 is an allele-specific suppressor. As tub2-404 mutants are also defective for spindle elongation, this provides additional support for a role for JSN1 during anaphase.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Mol Cell Biol. 1990 Oct;10(10):5295-304 - PubMed
    1. J Cell Sci. 1990 Oct;97 ( Pt 2):273-81 - PubMed
    1. New Biol. 1990 May;2(5):389-401 - PubMed
    1. Cell. 1991 Feb 22;64(4):827-39 - PubMed
    1. Methods Enzymol. 1991;194:319-29 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources