Physical linkage of the Vibrio cholerae mannose-sensitive hemagglutinin secretory and structural subunit gene loci: identification of the mshG coding sequence - PubMed (original) (raw)

Physical linkage of the Vibrio cholerae mannose-sensitive hemagglutinin secretory and structural subunit gene loci: identification of the mshG coding sequence

J W Marsh et al. Infect Immun. 1996 Feb.

Abstract

Vibrio cholerae O1 expresses a variety of cell surface factors which mediate bacterial adherence and colonization at the intestinal epithelium. The mannose-sensitive hemagglutinin (MSHA), a type IV pilus, is a potential attachment factor of the V. cholerae El Tor biotype. We describe a TnphoA mutant that is defective in its ability to hemagglutinate mouse erythrocytes. The TnphoA insertion maps to a recently identified genetic locus that encodes products that are predicted to be essential for assembly and export of the MSHA pilus. Insertional disruption at this locus in a mshA-phoA reporter strain provides evidence for a role of this locus in the latter stages of pilus assembly and/or export. These constructs have provided physical markers by which we have established close physical linkage of this secretion locus to a set of genes that includes the mshA structural gene. Sequence analysis of the intervening region between these two loci has revealed the presence of an open reading frame with homology to pilus biogenesis genes of several gram-negative bacteria. This genetic organization suggests an entire operon encoding the MSHA pilus and the components necessary for its assembly and secretion to the bacterial cell surface. The nomenclature of the MSHA structural and secretory locus has been redefined accordingly.

PubMed Disclaimer

Cited by

Genetic and transcriptional analyses of the Vibrio cholerae mannose-sensitive hemagglutinin type 4 pilus gene locus.
Marsh JW, Taylor RK. Marsh JW, et al. J Bacteriol. 1999 Feb;181(4):1110-7. doi: 10.1128/JB.181.4.1110-1117.1999. J Bacteriol. 1999. PMID: 9973335 Free PMC article.
Precisely Controlling Csr sRNA Levels by MshH Enhances Vibrio cholerae Colonization in Adult Mice.
Shi M, Ye J, Fan F, Zhao F, Zhong X, Zhong Z, Wang H, Wang Z, Yang M. Shi M, et al. Appl Environ Microbiol. 2023 Jul 26;89(7):e0056123. doi: 10.1128/aem.00561-23. Epub 2023 Jul 5. Appl Environ Microbiol. 2023. PMID: 37404138 Free PMC article.
The mannose-sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton.
Chiavelli DA, Marsh JW, Taylor RK. Chiavelli DA, et al. Appl Environ Microbiol. 2001 Jul;67(7):3220-5. doi: 10.1128/AEM.67.7.3220-3225.2001. Appl Environ Microbiol. 2001. PMID: 11425745 Free PMC article.
Type II secretion and pathogenesis.
Sandkvist M. Sandkvist M. Infect Immun. 2001 Jun;69(6):3523-35. doi: 10.1128/IAI.69.6.3523-3535.2001. Infect Immun. 2001. PMID: 11349009 Free PMC article. Review. No abstract available.
The Vibrio cholerae mannose-sensitive hemagglutinin is the receptor for a filamentous bacteriophage from V. cholerae O139.
Jouravleva EA, McDonald GA, Marsh JW, Taylor RK, Boesman-Finkelstein M, Finkelstein RA. Jouravleva EA, et al. Infect Immun. 1998 Jun;66(6):2535-9. doi: 10.1128/IAI.66.6.2535-2539.1998. Infect Immun. 1998. PMID: 9596713 Free PMC article.

References

1. J Bacteriol. 1989 Aug;171(8):4281-9 - PubMed
1. J Bacteriol. 1989 Apr;171(4):1870-8 - PubMed
1. J Infect Dis. 1990 Jun;161(6):1231-6 - PubMed
1. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
1. Infect Immun. 1991 Dec;59(12):4310-7 - PubMed

Physical linkage of the Vibrio cholerae mannose-sensitive hemagglutinin secretory and structural subunit gene loci: identification of the mshG coding sequence - PubMed (original) (raw)