Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases - PubMed (original) (raw)

Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases

Q L Yang et al. J Exp Med. 1996.

Abstract

The lipooligosaccharide (LOS) expressed by gonococci spontaneously varies its structure at high frequency, but the underlying genetic mechanism has not been described. We have previously reported that the genes encoding the glycosyl transferases responsible for the biosynthesis of the variable alpha chain of the LOS of Neisseria gonorrhoeae are located in a locus containing five genes, lgtA, lgtB, lgtC, lgtD, and lgtE. Sequence analysis showed that lgtA, lgtC, and lgtD contained poly-G tracts within the coding frames, leading to the hypothesis that shifts in the number of guanosine residues in the poly-G tracts might be responsible for the high frequency variation in structure of gonococcal LOS. We now provide experimental evidence confirming this hypothesis.

PubMed Disclaimer

Similar articles

• Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide.
  Gotschlich EC. Gotschlich EC. J Exp Med. 1994 Dec 1;180(6):2181-90. doi: 10.1084/jem.180.6.2181. J Exp Med. 1994. PMID: 7964493 Free PMC article.
• Genetic analysis of conservation and variation of lipooligosaccharide expression in two L8-immunotype strains of Neisseria meningitidis.
  Zhu P, Klutch MJ, Tsai CM. Zhu P, et al. FEMS Microbiol Lett. 2001 Sep 25;203(2):173-7. doi: 10.1111/j.1574-6968.2001.tb10837.x. FEMS Microbiol Lett. 2001. PMID: 11583844
• Phase variable changes in genes lgtA and lgtC within the lgtABCDE operon of Neisseria gonorrhoeae can modulate gonococcal susceptibility to normal human serum.
  Shafer WM, Datta A, Kolli VS, Rahman MM, Balthazar JT, Martin LE, Veal WL, Stephens DS, Carlson R. Shafer WM, et al. J Endotoxin Res. 2002;8(1):47-58. J Endotoxin Res. 2002. PMID: 11981445
• Urethral exudates of men with Neisseria gonorrhoeae infections select a restricted lipooligosaccharide phenotype during transmission.
  McLaughlin SE, Cheng H, Ghanem KG, Yang Z, Melendez J, Zenilman J, Griffiss JM. McLaughlin SE, et al. J Infect Dis. 2012 Oct;206(8):1227-32. doi: 10.1093/infdis/jis481. Epub 2012 Aug 16. J Infect Dis. 2012. PMID: 22904337 Free PMC article.

Cited by

• Implications of phase variation of a gene (pgtA) encoding a pilin galactosyl transferase in gonococcal pathogenesis.
  Banerjee A, Wang R, Supernavage SL, Ghosh SK, Parker J, Ganesh NF, Wang PG, Gulati S, Rice PA. Banerjee A, et al. J Exp Med. 2002 Jul 15;196(2):147-62. doi: 10.1084/jem.20012022. J Exp Med. 2002. PMID: 12119340 Free PMC article.
• Towards Computationally Guided Design and Engineering of a Neisseria meningitidis Serogroup W Capsule Polymerase with Altered Substrate Specificity.
  Paudel S, Wachira J, McCarthy PC. Paudel S, et al. Processes (Basel). 2021 Dec;9(12):2192. doi: 10.3390/pr9122192. Epub 2021 Dec 6. Processes (Basel). 2021. PMID: 37483532 Free PMC article.
• Analysis of lipooligosaccharide biosynthesis in the Neisseriaceae.
  Arking D, Tong Y, Stein DC. Arking D, et al. J Bacteriol. 2001 Feb;183(3):934-41. doi: 10.1128/JB.183.3.934-941.2001. J Bacteriol. 2001. PMID: 11208792 Free PMC article.
• A Novel Factor H-Fc Chimeric Immunotherapeutic Molecule against Neisseria gonorrhoeae.
  Shaughnessy J, Gulati S, Agarwal S, Unemo M, Ohnishi M, Su XH, Monks BG, Visintin A, Madico G, Lewis LA, Golenbock DT, Reed GW, Rice PA, Ram S. Shaughnessy J, et al. J Immunol. 2016 Feb 15;196(4):1732-40. doi: 10.4049/jimmunol.1500292. Epub 2016 Jan 15. J Immunol. 2016. PMID: 26773149 Free PMC article.
• Biochemical properties of Neisseria gonorrhoeae LgtE.
  Piekarowicz A, Stein DC. Piekarowicz A, et al. J Bacteriol. 2002 Dec;184(23):6410-6. doi: 10.1128/JB.184.23.6410-6416.2002. J Bacteriol. 2002. PMID: 12426326 Free PMC article.

References

1. 1. EMBO J. 1991 Jun;10(6):1307-15 - PubMed
2. 1. J Biol Chem. 1991 Oct 15;266(29):19303-11 - PubMed
3. 1. Biochemistry. 1991 Oct 29;30(43):10566-75 - PubMed
4. 1. Mol Immunol. 1991 Nov;28(11):1233-42 - PubMed
5. 1. J Exp Med. 1991 Dec 1;174(6):1601-5 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc