Cellular mechanisms underlying the increased duodenal iron absorption in rats in response to phenylhydrazine-induced haemolytic anaemia - PubMed (original) (raw)
Cellular mechanisms underlying the increased duodenal iron absorption in rats in response to phenylhydrazine-induced haemolytic anaemia
D K O'Riordan et al. Eur J Clin Invest. 1995 Oct.
Abstract
Haemolytic anaemia induced by phenylhydrazine (PZ) promotes iron absorption across rat small intestine. This present study investigates the role of the brush border potential difference (Vm) and mucosal reducing activity in the response. In addition, quantitative autoradiography was used to assess PZ-induced changes in the villus localization of brush border iron uptake. Iron transfer from duodenum to blood was increased significantly 5 days after treatment with PZ. Autoradiography showed that most brush border iron uptake occurred at the upper villus region and the maximal rate was increased fourfold by PZ. Duodenal villus length was increased in PZ-treated rats. PZ treatment did not influence mucosal reducing activity but Vm, measured using duodenal sheets, increased from -50 to -57 mV (P < 0.001) and this was due to a reduced brush border sodium permeability. Thus, an expanded absorptive surface and an enhanced electrical driving force for iron uptake across the duodenal brush border are important adaptations for increased iron absorption in PZ-induced haemolytic anaemia.
Comment in
- The use of ferric nitrilotriacetate and ferrozine in iron transport studies.
Marx JJ. Marx JJ. Eur J Clin Invest. 1995 Oct;25(10):721. doi: 10.1111/j.1365-2362.1995.tb01949.x. Eur J Clin Invest. 1995. PMID: 8557057 No abstract available.
Similar articles
- Mechanisms involved in increased iron uptake across rat duodenal brush-border membrane during hypoxia.
O'Riordan DK, Debnam ES, Sharp PA, Simpson RJ, Taylor EM, Srai SK. O'Riordan DK, et al. J Physiol. 1997 Apr 15;500 ( Pt 2)(Pt 2):379-84. doi: 10.1113/jphysiol.1997.sp022028. J Physiol. 1997. PMID: 9147325 Free PMC article. - Regional characteristics of intestinal iron absorption in the guinea-pig.
Chowrimootoo G, Debnam ES, Srai SK, Epstein O. Chowrimootoo G, et al. Exp Physiol. 1992 Jan;77(1):177-83. doi: 10.1113/expphysiol.1992.sp003571. Exp Physiol. 1992. PMID: 1543583 - Divalent metal inhibition of non-haem iron uptake across the rat duodenal brush border membrane.
Smith MW, Shenoy KB, Debnam ES, Dashwood MR, Churchill LJ, Srai SK. Smith MW, et al. Br J Nutr. 2002 Jul;88(1):51-6. doi: 10.1079/BJNBJN2002587. Br J Nutr. 2002. PMID: 12117427 - Ontogeny of iron uptake across brush border membrane of guinea pig duodenum and its autoradiographic localisation.
Debnam ES, Srai SK, Chowrimootoo G, Epstein O. Debnam ES, et al. Biol Neonate. 1991;59(1):30-6. doi: 10.1159/000243319. Biol Neonate. 1991. PMID: 2015325 - Structural and cellular adaptation of duodenal iron uptake in rats maintained on an iron-deficient diet.
Smith MW, Debnam ES, Dashwood MR, Srai SK. Smith MW, et al. Pflugers Arch. 2000 Feb;439(4):449-54. doi: 10.1007/s004249900193. Pflugers Arch. 2000. PMID: 10678741
Cited by
- Molecular mechanisms involved in intestinal iron absorption.
Sharp P, Srai SK. Sharp P, et al. World J Gastroenterol. 2007 Sep 21;13(35):4716-24. doi: 10.3748/wjg.v13.i35.4716. World J Gastroenterol. 2007. PMID: 17729393 Free PMC article. Review. - Intersection of Iron and Copper Metabolism in the Mammalian Intestine and Liver.
Doguer C, Ha JH, Collins JF. Doguer C, et al. Compr Physiol. 2018 Sep 14;8(4):1433-1461. doi: 10.1002/cphy.c170045. Compr Physiol. 2018. PMID: 30215866 Free PMC article. Review. - The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation.
Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, Kahn A, Vaulont S. Nicolas G, et al. J Clin Invest. 2002 Oct;110(7):1037-44. doi: 10.1172/JCI15686. J Clin Invest. 2002. PMID: 12370282 Free PMC article. - Duodenal mucosal reductase in wild-type and Hfe knockout mice on iron adequate, iron deficient, and iron rich feeding.
Simpson RJ, Debnam E, Beaumont N, Bahram S, Schümann K, Srai SK. Simpson RJ, et al. Gut. 2003 Apr;52(4):510-3. doi: 10.1136/gut.52.4.510. Gut. 2003. PMID: 12631660 Free PMC article. - Correlation between the expression of divalent metal transporter 1 and the content of hypoxia-inducible factor-1 in hypoxic HepG2 cells.
Li Z, Lai Z, Ya K, Fang D, Ho YW, Lei Y, Ming QZ. Li Z, et al. J Cell Mol Med. 2008 Apr;12(2):569-79. doi: 10.1111/j.1582-4934.2007.00145.x. J Cell Mol Med. 2008. PMID: 18419598 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical