Regulation of murine macrophage IL-12 production. Activation of macrophages in vivo, restimulation in vitro, and modulation by other cytokines - PubMed (original) (raw)

. 1996 Feb 1;156(3):1196-206.

Affiliations

PMID: 8557998

Regulation of murine macrophage IL-12 production. Activation of macrophages in vivo, restimulation in vitro, and modulation by other cytokines

M J Skeen et al. J Immunol. 1996.

Abstract

IL-12 is important in the host response to a variety of pathogens. It plays an adjuvant-like role in an initial immune response as well as a therapeutic role in established infections. Despite its well documented importance, comparatively little is known about the regulation of IL-12 production. In this study, we examined IL-12 production by cultured murine peritoneal macrophages from two perspectives: 1) macrophage activation in vivo, and 2) stimulation of IL-12 secretion in vitro. Macrophages were maximally activated within 48 h in vivo during infection with Listeria. Interestingly, although avirulent or heat-killed Listeria induced only minimal production of IL-12 by macrophages, the immunogenic combination of heat-killed bacteria and rIL-12 was highly stimulatory for IL-12 production. LPS and peritoneal inflammatory agents were also stimulatory, but latex beads were ineffective, indicating that microbial components were essential and phagocytosis alone was insufficient. Restimulation in vitro revealed similar patterns, in that infection and LPS were stimulatory but latex beads were not. A systematic survey of potential stimulatory agents showed that microbial heat shock proteins, crude bacterial extracts, bacterial superantigens, a yeast extract, and dsRNA induced IL-12 in vitro. Other cytokines also influenced IL-12 induction. IFN-gamma, which is up-regulated during infection, acted in synergy with other stimuli, suggesting an amplification loop for IL-12 production, whereas IL-4, IL-10, IL-13, and TGF-beta were inhibitory. The existence of a broad range of stimuli from a wide variety of pathogenic organisms underscores the fundamental importance of IL-12 in host defense.

PubMed Disclaimer

Cited by

Characteristics of the Gut Microbiome and IL-13/TGF-β1 Mediated Fibrosis in Post-Kasai Cholangitis of Biliary Atresia.
Meng L, Liu J, Wang J, Du M, Zhang S, Huang Y, Shen Z, Dong R, Chen G, Zheng S. Meng L, et al. Front Pediatr. 2021 Nov 8;9:751204. doi: 10.3389/fped.2021.751204. eCollection 2021. Front Pediatr. 2021. PMID: 34858903 Free PMC article.
Reparative and Maladaptive Inflammation in Tendon Healing.
Arvind V, Huang AH. Arvind V, et al. Front Bioeng Biotechnol. 2021 Jul 19;9:719047. doi: 10.3389/fbioe.2021.719047. eCollection 2021. Front Bioeng Biotechnol. 2021. PMID: 34350166 Free PMC article. Review.
Armored CAR T-Cells: The Next Chapter in T-Cell Cancer Immunotherapy.
Hawkins ER, D'Souza RR, Klampatsa A. Hawkins ER, et al. Biologics. 2021 Apr 14;15:95-105. doi: 10.2147/BTT.S291768. eCollection 2021. Biologics. 2021. PMID: 33883875 Free PMC article. Review.
Lymph Node Stromal Cells From Different Draining Areas Distinctly Regulate the Development of Chronic Intestinal Inflammation.
Basic M, Peppermüller PP, Bolsega S, Bleich A, Bornemann M, Bode U, Buettner M. Basic M, et al. Front Immunol. 2021 Feb 16;11:549473. doi: 10.3389/fimmu.2020.549473. eCollection 2020. Front Immunol. 2021. PMID: 33664727 Free PMC article.
Attenuation of intestinal inflammation in IL-10 deficient mice by a plasmid carrying Lactococcus lactis strain.
Zurita-Turk M, Mendes Souza B, Prósperi de Castro C, Bastos Pereira V, Pecini da Cunha V, Melo Preisser T, Caetano de Faria AM, Carmona Cara Machado D, Miyoshi A. Zurita-Turk M, et al. BMC Biotechnol. 2020 Jul 23;20(1):38. doi: 10.1186/s12896-020-00631-0. BMC Biotechnol. 2020. PMID: 32703192 Free PMC article.

Regulation of murine macrophage IL-12 production. Activation of macrophages in vivo, restimulation in vitro, and modulation by other cytokines - PubMed (original) (raw)