Heightened inflammatory response and cytokine expression in vivo to cagA+ Helicobacter pylori strains - PubMed (original) (raw)
Affiliations
- PMID: 8558837
Heightened inflammatory response and cytokine expression in vivo to cagA+ Helicobacter pylori strains
R M Peek Jr et al. Lab Invest. 1995 Dec.
Abstract
Background: Helicobacter pylori strains that possess the cytotoxin-associated gene (cagA) are highly associated with peptic ulcer disease, but the role of cagA in pathogenesis is unknown.
Experimental design: To test the hypothesis that cagA+ stains elicit a greater proinflammatory cytokine response in the gastric mucosa than cagA- strains, gastric biopsies were obtained from 52 patients and studied by histology, culture, enzyme-linked immunosorbent assay, and reverse transcription polymerase chain reaction.
Results: Of 52 patients, 32 (62%) were infected with H. pylori based upon both serology and histology or culture, 16 (31%) were negative by serology, histology, and culture, and four (7%) were positive by serology only. Of 15 H. pylori-infected patients with peptic ulceration, 14 (92%) were infected with cagA+ strains compared with 8 (50%) of 16 patients with gastritis alone, and those infected with cagA+ strains had significantly higher grades of inflammation in the gastric mucosa. Antral inflammation score was significantly associated with IL-8 production. Antral biopsies from infected patients, compared with uninfected patients, significantly more often demonstrated IL-1 beta, IL-2, and IL-8 expression, and those infected with cagA+ compared with cagA- strains significantly more often expressed IL-1 alpha and IL-1 beta and showed elevated antral IL-8 protein levels. Similarly, patients with ulcer disease significantly more often expressed antral IL-1 alpha and IL-8 than those without ulceration.
Conclusion: These results indicate that infection with cagA+ H. pylori strains is associated with higher grades of gastric inflammation, correlating with enhanced mucosal levels of IL-8, and increased risk of peptic ulceration.
Comment in
- Helicobacter pylori and gastric mucosal cytokines: evidence that CagA-positive strains are more virulent.
Crabtree JE, Farmery SM. Crabtree JE, et al. Lab Invest. 1995 Dec;73(6):742-5. Lab Invest. 1995. PMID: 8558835 No abstract available.
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