Antiproliferative properties of the USF family of helix-loop-helix transcription factors - PubMed (original) (raw)

Antiproliferative properties of the USF family of helix-loop-helix transcription factors

X Luo et al. Proc Natl Acad Sci U S A. 1996.

Abstract

USF is a family of transcription factors characterized by a highly conserved basic-helix-loop-helix-leucine zipper (bHLH-zip) DNA-binding domain. Two different USF genes, termed USF1 and USF2, are ubiquitously expressed in both humans and mice. The USF1 and USF2 proteins contain highly divergent transcriptional activation domains but share extensive homologies in the bHLH-zip region and recognize the same CACGTG DNA motifs. Although the DNA-binding and transcriptional activities of these proteins have been characterized, the biological function of USF is not well understood. Here, focus- and colony-formation assays were used to investigate the potential involvement of USF in the regulation of cellular transformation and proliferation. Both USF1 and USF2 inhibited the transformation of rat embryo fibroblasts mediated by Ras and c-Myc, a bHLH-zip transcription factor that also binds CACGTG motifs. DNA binding was required but not fully sufficient for inhibition of Myc-dependent transformation by USF, since deletion mutants containing only the DNA-binding domains of USF1 or USF2 produced partial inhibition. While the effect of USF1 was selective for Myc-dependent transformation, wild-type USF2 exerted in addition a strong inhibition of E1A-mediated transformation and a strong suppression of HeLa cell colony formation. These results suggest that members of the USF family may serve as negative regulators of cellular proliferation in two ways, one by antagonizing the transforming function of Myc, the other through a more general growth-inhibitory effect.

PubMed Disclaimer

Cited by

Human papillomavirus type 16 E6 activates TERT gene transcription through induction of c-Myc and release of USF-mediated repression.
McMurray HR, McCance DJ. McMurray HR, et al. J Virol. 2003 Sep;77(18):9852-61. doi: 10.1128/jvi.77.18.9852-9861.2003. J Virol. 2003. PMID: 12941894 Free PMC article.
Integration of proteomic and transcriptomic profiles identifies a novel PDGF-MYC network in human smooth muscle cells.
Yang W, Ramachandran A, You S, Jeong H, Morley S, Mulone MD, Logvinenko T, Kim J, Hwang D, Freeman MR, Adam RM. Yang W, et al. Cell Commun Signal. 2014 Aug 1;12:44. doi: 10.1186/s12964-014-0044-z. Cell Commun Signal. 2014. PMID: 25080971 Free PMC article.
Max is acetylated by p300 at several nuclear localization residues.
Faiola F, Wu YT, Pan S, Zhang K, Farina A, Martinez E. Faiola F, et al. Biochem J. 2007 May 1;403(3):397-407. doi: 10.1042/BJ20061593. Biochem J. 2007. PMID: 17217336 Free PMC article.
Myc: a single gene controls both proliferation and apoptosis in mammalian cells.
Desbarats L, Schneider A, Müller D, Bürgin A, Eilers M. Desbarats L, et al. Experientia. 1996 Dec 15;52(12):1123-9. doi: 10.1007/BF01952111. Experientia. 1996. PMID: 8988255 Review.
Functional interplay between the transcription factors USF1 and PDX-1 and protein kinase CK2 in pancreatic β-cells.
Spohrer S, Groß R, Nalbach L, Schwind L, Stumpf H, Menger MD, Ampofo E, Montenarh M, Götz C. Spohrer S, et al. Sci Rep. 2017 Nov 27;7(1):16367. doi: 10.1038/s41598-017-16590-0. Sci Rep. 2017. PMID: 29180680 Free PMC article.

References

1. Nature. 1982 Apr 1;296(5856):404-9 - PubMed
1. Mol Cell Biol. 1994 Jun;14(6):4032-43 - PubMed
1. EMBO J. 1994 Sep 1;13(17):4070-9 - PubMed
1. EMBO J. 1994 Sep 15;13(18):4291-301 - PubMed
1. J Biol Chem. 1994 Sep 30;269(39):23894-903 - PubMed

Antiproliferative properties of the USF family of helix-loop-helix transcription factors - PubMed (original) (raw)