Transposase is the only nematode protein required for in vitro transposition of Tc1 - PubMed (original) (raw)

. 1996 Mar 15;10(6):755-61.

doi: 10.1101/gad.10.6.755.

Affiliations

• PMID: 8598301
• DOI: 10.1101/gad.10.6.755

Free article

Transposase is the only nematode protein required for in vitro transposition of Tc1

J C Vos et al. Genes Dev. 1996.

Free article

Abstract

The Tc1 element of Caenorhabditis elegans is a member of the most widespread class of DNA transposons known in nature. Here, we describe efficient and precise transposition of Tc1 in a cell-free system. Tc1 appears to jump by a cut-and-paste mechanism of transposition. The terminal 26 bp of the Tc1 terminal repeats together with the flanking TA sequence are sufficient for transposition. The target site choice in vitro is similar to that in vivo. Transposition is achieved with an extract prepared from nuclei of transgenic nematodes that overexpress Tc1 transposase but also by recombinant transposase purified from Escherichia coli. The simple reaction requirements explain why horizontal spread of Tc1/mariner transposons can occur. They also suggest that Tcl may be a good vector for transgenesis of diverse animal species.

PubMed Disclaimer

Similar articles

• Tc7, a Tc1-hitch hiking transposon in Caenorhabditis elegans.
  Rezsohazy R, van Luenen HG, Durbin RM, Plasterk RH. Rezsohazy R, et al. Nucleic Acids Res. 1997 Oct 15;25(20):4048-54. doi: 10.1093/nar/25.20.4048. Nucleic Acids Res. 1997. PMID: 9321656 Free PMC article.
• Characterization of the Caenorhabditis elegans Tc1 transposase in vivo and in vitro.
  Vos JC, van Luenen HG, Plasterk RH. Vos JC, et al. Genes Dev. 1993 Jul;7(7A):1244-53. doi: 10.1101/gad.7.7a.1244. Genes Dev. 1993. PMID: 8391505
• Transposon Tc1 of the nematode Caenorhabditis elegans jumps in human cells.
  Schouten GJ, van Luenen HG, Verra NC, Valerio D, Plasterk RH. Schouten GJ, et al. Nucleic Acids Res. 1998 Jun 15;26(12):3013-7. doi: 10.1093/nar/26.12.3013. Nucleic Acids Res. 1998. PMID: 9611249 Free PMC article.
• Assembly of the Tc1 and mariner transposition initiation complexes depends on the origins of their transposase DNA binding domains.
  Brillet B, Bigot Y, Augé-Gouillou C. Brillet B, et al. Genetica. 2007 Jun;130(2):105-20. doi: 10.1007/s10709-006-0025-2. Epub 2006 Aug 16. Genetica. 2007. PMID: 16912840 Review.
• IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): functions deduced from their DNA-binding ability.
  Sekino N, Sekine Y, Ohtsubo E. Sekino N, et al. Adv Biophys. 1995;31:209-22. doi: 10.1016/0065-227x(95)99393-4. Adv Biophys. 1995. PMID: 7625275 Review.

Cited by

• P Transposable Elements in Drosophila and other Eukaryotic Organisms.
  Majumdar S, Rio DC. Majumdar S, et al. Microbiol Spectr. 2015 Apr;3(2):MDNA3-0004-2014. doi: 10.1128/microbiolspec.MDNA3-0004-2014. Microbiol Spectr. 2015. PMID: 26104714 Free PMC article. Review.
• DNA-binding activity and subunit interaction of the mariner transposase.
  Zhang L, Dawson A, Finnegan DJ. Zhang L, et al. Nucleic Acids Res. 2001 Sep 1;29(17):3566-75. doi: 10.1093/nar/29.17.3566. Nucleic Acids Res. 2001. PMID: 11522826 Free PMC article.
• The mariner Mos1 transposase produced in tobacco is active in vitro.
  Thomas X, Hedhili S, Beuf L, Demattéi MV, Laparra H, Khong GN, Breitler JC, Montandon F, Carnus E, Norre F, Burtin D, Gantet P, Bigot Y, Renault S. Thomas X, et al. Genetica. 2010 May;138(5):519-30. doi: 10.1007/s10709-009-9414-7. Epub 2009 Oct 22. Genetica. 2010. PMID: 19847655
• High frequencies of Minos transposon mobilization are obtained in insects by using in vitro synthesized mRNA as a source of transposase.
  Kapetanaki MG, Loukeris TG, Livadaras I, Savakis C. Kapetanaki MG, et al. Nucleic Acids Res. 2002 Aug 1;30(15):3333-40. doi: 10.1093/nar/gkf455. Nucleic Acids Res. 2002. PMID: 12140317 Free PMC article.
• In vitro transposition of ISY100, a bacterial insertion sequence belonging to the Tc1/mariner family.
  Feng X, Colloms SD. Feng X, et al. Mol Microbiol. 2007 Sep;65(6):1432-43. doi: 10.1111/j.1365-2958.2007.05842.x. Epub 2007 Aug 6. Mol Microbiol. 2007. PMID: 17680987 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • HighWire

• Other Literature Sources

  • The Lens - Patent Citations Database