Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast - PubMed (original) (raw)

Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast

F He et al. RNA. 1996 Feb.

Abstract

Rapid turnover of nonsense-containing mRNAs in the yeast Saccharomyces cerevisiae is dependent on the products of the UPF1 (Upf1p), NMD2/UPF2 (Nmd2p) and UPF3 (Upf3p) genes. Mutations in each of these genes lead to the selective stabilization of mRNAs containing early nonsense mutations without affecting the decay rates of most other mRNAs. NMD2 was recently identified in a two-hybrid screen as a gene that encodes a Upf1p-interacting protein. To identify the amino acids essential to this interaction, we used two-hybrid analysis as well as missense, nonsense, and deletion mutants of NMD2, and mapped the Upf1p-interacting domain of Nmd2p to a 157-amino acid segment at its C-terminus. Mutations in this domain that disrupt interaction with Upf1p also disrupt nonsense-mediated mRNA decay. A dominant-negative deletion allele of NMD2 identified previously includes the Upf1p-interacting domain. However, mutations in the Upf1p-interacting domain do not affect dominant-negative inhibition of mRNA decay caused by this allele, suggesting interaction with yet another factor. These results, and the observation that deletion of a putative nuclear localization signal and a putative transmembrane domain also inactivate nonsense-mediated mRNA decay, suggest that Nmd2p may contain as many as four important functional domains.

PubMed Disclaimer

Cited by

Nonsense-mediated mRNA decay mutation in Aspergillus nidulans.
Morozov IY, Negrete-Urtasun S, Tilburn J, Jansen CA, Caddick MX, Arst HN Jr. Morozov IY, et al. Eukaryot Cell. 2006 Nov;5(11):1838-46. doi: 10.1128/EC.00220-06. Epub 2006 Sep 8. Eukaryot Cell. 2006. PMID: 16963627 Free PMC article.
Telomere length regulation and telomeric chromatin require the nonsense-mediated mRNA decay pathway.
Lew JE, Enomoto S, Berman J. Lew JE, et al. Mol Cell Biol. 1998 Oct;18(10):6121-30. doi: 10.1128/MCB.18.10.6121. Mol Cell Biol. 1998. PMID: 9742129 Free PMC article.
Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4).
Serin G, Gersappe A, Black JD, Aronoff R, Maquat LE. Serin G, et al. Mol Cell Biol. 2001 Jan;21(1):209-23. doi: 10.1128/MCB.21.1.209-223.2001. Mol Cell Biol. 2001. PMID: 11113196 Free PMC article.
Interactions between Upf1 and the decapping factors Edc3 and Pat1 in Saccharomyces cerevisiae.
Swisher KD, Parker R. Swisher KD, et al. PLoS One. 2011;6(10):e26547. doi: 10.1371/journal.pone.0026547. Epub 2011 Oct 31. PLoS One. 2011. PMID: 22065998 Free PMC article.
Nonsense-Mediated mRNA Decay: Degradation of Defective Transcripts Is Only Part of the Story.
He F, Jacobson A. He F, et al. Annu Rev Genet. 2015;49:339-66. doi: 10.1146/annurev-genet-112414-054639. Epub 2015 Oct 2. Annu Rev Genet. 2015. PMID: 26436458 Free PMC article. Review.

References

1. Nature. 1969 Oct 25;224(5217):329-31 - PubMed
1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
1. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134-7 - PubMed
1. Genetics. 1980 Aug;95(4):833-53 - PubMed
1. Mol Cell Biol. 1988 May;8(5):2159-65 - PubMed

Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast - PubMed (original) (raw)