Autoantibodies inhibit interleukin-7-mediated proliferation and are associated with the age-dependent loss of pre-B cells in autoimmune New Zealand Black Mice - PubMed (original) (raw)
. 1996 Apr 15;87(8):3289-96.
Affiliations
- PMID: 8605345
Free article
Autoantibodies inhibit interleukin-7-mediated proliferation and are associated with the age-dependent loss of pre-B cells in autoimmune New Zealand Black Mice
M S Merchant et al. Blood. 1996.
Free article
Abstract
Surface IgM+B220+ B cell precursors can be categorized as either leukosialin (CD43/S7) negative (late stage pre-B cells) or positive (pro-B/early pre-B cells). In autoimmune New Zealand Black (NZB) mice, bone marrow small pre-B cells (IgM-CD43-B220+) and pro-B/early pre-B cells (IgM-CD43+B220+) declined significantly with age. In particular, subpopulations of pro-B/early pre-B cells expressing the heat stable antigen (HSA) were found in lower proportions with age. Significant decreases in interleukin-7 (IL-7) colony forming units (CFU) were also seen in NZB mice by 6 to 8 months of age and accompanied alterations in the numbers of pro-B and pre-B cells in bone marrow. Concomitant with reduced numbers of B lineage precursor cells and IL-7 CFU in vivo, NZB mice produced serum IgM antibodies that strongly inhibited IL-7 CFU responses in vitro. Two monoclonal IgM antibodies (5G9, 2F5) derived from LPS stimulated 10-month-old NZB splenocytes recognized pre-B cell surface antigens on both pre-B cell lines and on IL-7 stimulated bone marrow pro-B/pre-B cells. However, these monoclonal antibodies (MoAb) failed to significantly stain ex vivo bone marrow cells. The 5G9 and 2F5 MoAbs also partially inhibited IL-7 CFU in vitro. These results suggest that NZB bone marrow becomes increasingly deficient in B cell precursors and especially in IL-7 responsive pre-B cells with age. IgM serum antibodies and monoclonal IgM antibodies derived from older NZB mice inhibit pre-B cell growth to IL-7. The production of such autoantibodies may interfere with B cell development in aging NZB mice by preventing IL-7-mediated proliferation.
Similar articles
- Abnormalities of B lineage cells are demonstrable in long term lymphoid bone marrow cultures of New Zealand black mice.
Yoshida S, Dorshkind K, Bearer E, Castles JJ, Ahmed A, Gershwin ME. Yoshida S, et al. J Immunol. 1987 Sep 1;139(5):1454-8. J Immunol. 1987. PMID: 3497971 - Bone marrow cells from young and old New Zealand black mice can reconstitute B lymphocytes in severe combined immunodeficient recipients.
Dorshkind K, Yoshida S, Gershwin ME. Dorshkind K, et al. J Autoimmun. 1989 Apr;2(2):173-86. doi: 10.1016/0896-8411(89)90153-4. J Autoimmun. 1989. PMID: 2669797 - Natural IgM anti-leucocyte autoantibodies (IgM-ALA) regulate inflammation induced by innate and adaptive immune mechanisms.
Lobo PI, Brayman KL, Okusa MD. Lobo PI, et al. J Clin Immunol. 2014 Jul;34 Suppl 1(0 1):S22-9. doi: 10.1007/s10875-014-0027-2. Epub 2014 Apr 8. J Clin Immunol. 2014. PMID: 24711004 Free PMC article. Review. - Natural IgM: beneficial autoantibodies for the control of inflammatory and autoimmune disease.
Grönwall C, Silverman GJ. Grönwall C, et al. J Clin Immunol. 2014 Jul;34 Suppl 1(0 1):S12-21. doi: 10.1007/s10875-014-0025-4. Epub 2014 Apr 2. J Clin Immunol. 2014. PMID: 24691998 Free PMC article. Review.
Cited by
- Neutrophil-mediated IFN activation in the bone marrow alters B cell development in human and murine systemic lupus erythematosus.
Palanichamy A, Bauer JW, Yalavarthi S, Meednu N, Barnard J, Owen T, Cistrone C, Bird A, Rabinovich A, Nevarez S, Knight JS, Dedrick R, Rosenberg A, Wei C, Rangel-Moreno J, Liesveld J, Sanz I, Baechler E, Kaplan MJ, Anolik JH. Palanichamy A, et al. J Immunol. 2014 Feb 1;192(3):906-18. doi: 10.4049/jimmunol.1302112. Epub 2013 Dec 30. J Immunol. 2014. PMID: 24379124 Free PMC article. - In senescence, age-associated B cells secrete TNFα and inhibit survival of B-cell precursors.
Ratliff M, Alter S, Frasca D, Blomberg BB, Riley RL. Ratliff M, et al. Aging Cell. 2013 Apr;12(2):303-11. doi: 10.1111/acel.12055. Aging Cell. 2013. PMID: 23410004 Free PMC article. - Ageing, autoimmunity and arthritis: senescence of the B cell compartment - implications for humoral immunity.
Johnson SA, Cambier JC. Johnson SA, et al. Arthritis Res Ther. 2004;6(4):131-9. doi: 10.1186/ar1180. Epub 2004 May 10. Arthritis Res Ther. 2004. PMID: 15225355 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
Research Materials