Molecular single cell analysis demonstrates the derivation of a peripheral blood-derived cell line (L1236) from the Hodgkin/Reed-Sternberg cells of a Hodgkin's lymphoma patient - PubMed (original) (raw)
Case Reports
. 1996 Apr 15;87(8):3429-36.
Affiliations
- PMID: 8605361
Free article
Case Reports
Molecular single cell analysis demonstrates the derivation of a peripheral blood-derived cell line (L1236) from the Hodgkin/Reed-Sternberg cells of a Hodgkin's lymphoma patient
H Kanzler et al. Blood. 1996.
Free article
Abstract
A novel cell line, L1236, was established from the peripheral blood of a patient with Hodgkin's disease (HD). Two Ig VH and one Vkappa gene rearrangements were amplified by polymerase chain reaction (PCR) from the DNA of this cell line, demonstrating its derivation from the B-cell lineage. To test the cell line for its clonal relationship to the Hodgkin/Reed-Sternberg (H-RS) cells in the patient, single H-RS cells were micromanipulated from tissue sections of a tumor-infiltrated bone marrow specimen from that patient and analyzed by PCR for Ig gene rearrangements. The same rearrangements detected in the cell line were also found repeatedly in H-RS cells of the biopsy material. Thus, L1236 is the first cell line established from a case of HD for which a derivation from the H-RS cells of the patient could be demonstrated. Furthermore, the selective isolation of identical V gene rearrangements from multiple single H-RS cells demonstrates that these cells represented a clonal population in the patient.
Similar articles
- Detection of clonal Hodgkin and Reed-Sternberg cells with identical somatically mutated and rearranged VH genes in different biopsies in relapsed Hodgkin's disease.
Vockerodt M, Soares M, Kanzler H, Küppers R, Kube D, Hansmann ML, Diehl V, Tesch H. Vockerodt M, et al. Blood. 1998 Oct 15;92(8):2899-907. Blood. 1998. PMID: 9763576 - Peripheral blood mononuclear cells of a patient with advanced Hodgkin's lymphoma give rise to permanently growing Hodgkin-Reed Sternberg cells.
Wolf J, Kapp U, Bohlen H, Kornacker M, Schoch C, Stahl B, Mücke S, von Kalle C, Fonatsch C, Schaefer HE, Hansmann ML, Diehl V. Wolf J, et al. Blood. 1996 Apr 15;87(8):3418-28. Blood. 1996. PMID: 8605360 - Hodgkin's disease: clonal Ig gene rearrangements in Hodgkin and Reed-Sternberg cells picked from histological sections.
Küppers R, Rajewsky K, Zhao M, Simons G, Laumann R, Fischer R, Hansmann ML. Küppers R, et al. Ann N Y Acad Sci. 1995 Sep 29;764:523-4. doi: 10.1111/j.1749-6632.1995.tb55877.x. Ann N Y Acad Sci. 1995. PMID: 7486580 No abstract available. - Clonality and germinal centre B-cell derivation of Hodgkin/Reed-Sternberg cells in Hodgkin's disease.
Küppers R, Hansmann ML, Rajewsky K. Küppers R, et al. Ann Oncol. 1998;9 Suppl 5:S17-20. doi: 10.1093/annonc/9.suppl_5.s17. Ann Oncol. 1998. PMID: 9926232 Review. - Single cell analysis of H/RS cells.
Chan WC, Delabie J. Chan WC, et al. Ann Oncol. 1996;7 Suppl 4:41-3. doi: 10.1093/annonc/7.suppl_4.s41. Ann Oncol. 1996. PMID: 8836408 Review.
Cited by
- Identification of special AT-rich sequence binding protein 1 as a novel tumor antigen recognized by CD8+ T cells: implication for cancer immunotherapy.
Wang M, Yin B, Matsueda S, Deng L, Li Y, Zhao W, Zou J, Li Q, Loo C, Wang RF, Wang HY. Wang M, et al. PLoS One. 2013;8(2):e56730. doi: 10.1371/journal.pone.0056730. Epub 2013 Feb 21. PLoS One. 2013. PMID: 23437226 Free PMC article. - Hodgkin and Reed-Sternberg cells in Hodgkin's disease represent the outgrowth of a dominant tumor clone derived from (crippled) germinal center B cells.
Kanzler H, Küppers R, Hansmann ML, Rajewsky K. Kanzler H, et al. J Exp Med. 1996 Oct 1;184(4):1495-505. doi: 10.1084/jem.184.4.1495. J Exp Med. 1996. PMID: 8879220 Free PMC article. - [Pathogenesis and therapy of Hodgkin lymphoma].
Tesch H, Bohlen H, Wolf J, Engert A. Tesch H, et al. Med Klin (Munich). 1998 Feb 15;93(2):82-90. doi: 10.1007/BF03043282. Med Klin (Munich). 1998. PMID: 9545706 Review. German. - Chromosomal Instability in Hodgkin Lymphoma: An In-Depth Review and Perspectives.
Cuceu C, Hempel WM, Sabatier L, Bosq J, Carde P, M'kacher R. Cuceu C, et al. Cancers (Basel). 2018 Mar 26;10(4):91. doi: 10.3390/cancers10040091. Cancers (Basel). 2018. PMID: 29587466 Free PMC article. Review. - Effective therapy of murine models of human leukemia and lymphoma with radiolabeled anti-CD30 antibody, HeFi-1.
Zhang M, Yao Z, Patel H, Garmestani K, Zhang Z, Talanov VS, Plascjak PS, Goldman CK, Janik JE, Brechbiel MW, Waldmann TA. Zhang M, et al. Proc Natl Acad Sci U S A. 2007 May 15;104(20):8444-8. doi: 10.1073/pnas.0702496104. Epub 2007 May 8. Proc Natl Acad Sci U S A. 2007. PMID: 17488826 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical