Generation of human dendritic cells/Langerhans cells from circulating CD34+ hematopoietic progenitor cells - PubMed (original) (raw)

. 1996 Feb 15;87(4):1292-302.

Affiliations

• PMID: 8608217

Free article

Generation of human dendritic cells/Langerhans cells from circulating CD34+ hematopoietic progenitor cells

D Strunk et al. Blood. 1996.

Free article

Abstract

Human Langerhans cells (LC) are CD1a+ dendritic cells (DC) that function as potent antigen-presenting cells for primary and secondary immune responses. Limitations in DC/LC numbers, imposed by difficult and tedious isolation procedures, have so far precluded their use as immunogens in the generation and/or augmentation of host responses against various pathogens. Therefore, we have developed a procedure for the generation of human DC/LC from CD34+ hematopoietic progenitor cells (HPC) isolated (mean: 0.7 x 10(6)/ buffy coat and 2.6 x 10(6)/leukapheresis product) and purified ( > 95%) from the peripheral blood of healthy adults. In vitro stimulation of these cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha led to their vigorous proliferation and differentiation resulting in the emergence of CD45+/CD68+/CD3-/CD19-/CD56- leukocytes some of which (mean: 12%) express CD1a and exhibit anti-CD4 and anti-major histocompatibility complex (MHC) class II reactivity. These CD1a- leukocytes include (1) LC as evidenced by the presence of Birbeck granules (BG), (2) CD14+ monocytes, and (3) Birbeck granule-negative cells with a dendritic morphology. Addition of interleukin (IL)-4 to the cytokine cocktail interfered with the development of monocytes and led to a reduction in the overall yield but, on the other hand, resulted in an increased percentage of CD1a+ cells (mean: 24%) among all cells generated. In vitro generated CD1a+, but not CD1a- HPC-derived cells are potent stimulators of the primary mixed leukocyte reaction and, as such, promising candidates for vaccination purposes.

PubMed Disclaimer

Similar articles

• Delineation of the dendritic cell lineage by generating large numbers of Birbeck granule-positive Langerhans cells from human peripheral blood progenitor cells in vitro.
  Mackensen A, Herbst B, Köhler G, Wolff-Vorbeck G, Rosenthal FM, Veelken H, Kulmburg P, Schaefer HE, Mertelsmann R, Lindemann A. Mackensen A, et al. Blood. 1995 Oct 1;86(7):2699-707. Blood. 1995. PMID: 7545468
• Respective involvement of TGF-beta and IL-4 in the development of Langerhans cells and non-Langerhans dendritic cells from CD34+ progenitors.
  Caux C, Massacrier C, Dubois B, Valladeau J, Dezutter-Dambuyant C, Durand I, Schmitt D, Saeland S. Caux C, et al. J Leukoc Biol. 1999 Nov;66(5):781-91. doi: 10.1002/jlb.66.5.781. J Leukoc Biol. 1999. PMID: 10577510
• In vitro differentiation of CD34+ hematopoietic progenitor cells toward distinct dendritic cell subsets of the birbeck granule and MIIC-positive Langerhans cell and the interdigitating dendritic cell type.
  Herbst B, Köhler G, Mackensen A, Veelken H, Kulmburg P, Rosenthal FM, Schaefer HE, Mertelsmann R, Fisch P, Lindemann A. Herbst B, et al. Blood. 1996 Oct 1;88(7):2541-8. Blood. 1996. PMID: 8839846
• [The Langerhans cell: from in vitro production to use in cellular immunotherapy].
  Schmitt D. Schmitt D. J Soc Biol. 2001;195(1):69-74. J Soc Biol. 2001. PMID: 11530504 Review. French.

Cited by

• Production of functional dendritic cells from menstrual blood--a new dendritic cell source for immune therapy.
  Phuc PV, Lam DH, Ngoc VB, Thu DT, Nguyet NT, Ngoc PK. Phuc PV, et al. In Vitro Cell Dev Biol Anim. 2011 Jun;47(5-6):368-75. doi: 10.1007/s11626-011-9399-2. Epub 2011 Mar 18. In Vitro Cell Dev Biol Anim. 2011. PMID: 21424240
• Efficient human cytomegalovirus reactivation is maturation dependent in the Langerhans dendritic cell lineage and can be studied using a CD14+ experimental latency model.
  Huang MM, Kew VG, Jestice K, Wills MR, Reeves MB. Huang MM, et al. J Virol. 2012 Aug;86(16):8507-15. doi: 10.1128/JVI.00598-12. Epub 2012 May 30. J Virol. 2012. PMID: 22647696 Free PMC article.
• Cell(s) of Origin of Langerhans Cell Histiocytosis.
  Collin M, Bigley V, McClain KL, Allen CE. Collin M, et al. Hematol Oncol Clin North Am. 2015 Oct;29(5):825-38. doi: 10.1016/j.hoc.2015.06.003. Epub 2015 Aug 20. Hematol Oncol Clin North Am. 2015. PMID: 26461145 Free PMC article. Review.
• Dynamics of Human Cytomegalovirus Infection in CD34+ Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells.
  Coronel R, Takayama S, Juwono T, Hertel L. Coronel R, et al. J Virol. 2015 May;89(10):5615-32. doi: 10.1128/JVI.00305-15. Epub 2015 Mar 11. J Virol. 2015. PMID: 25762731 Free PMC article.
• HIV-1 infection of placental cord blood monocyte-derived dendritic cells.
  Folcik RM, Merrill JD, Li Y, Guo CJ, Douglas SD, Starr SE, Ho WZ. Folcik RM, et al. J Hematother Stem Cell Res. 2001 Oct;10(5):609-20. doi: 10.1089/152581601753193823. J Hematother Stem Cell Res. 2001. PMID: 11672507 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Medical

  • MedlinePlus Health Information

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal