Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide - PubMed (original) (raw)
Comparative Study
Formation of chimeric DNA primer extension products by template switching onto an annealed downstream oligonucleotide
R Patel et al. Proc Natl Acad Sci U S A. 1996.
Abstract
Oligodeoxynucleotide sequences are described that anneal to a template downstream of a priming site. During polymerase-catalyzed extension of the primer, the extending primer shifts from the original template to a segment of the annealed oligonucleotide that acts as an alternative template. The resulting chimeric extended primer has one segment that is complementary to the template and a second segment that is complementary to the oligonucleotide. The influence of the sequence elements of the oligonucleotide and the reaction conditions on template switching have been explored. The sequence requirements for template switching are compared to those for transposon excision.
References
- J Biol Chem. 1971 Apr 25;246(8):2692-701 - PubMed
- Mol Biol Biochem Biophys. 1977;24:191-218 - PubMed
- J Mol Biol. 1978 Dec 25;126(4):847-57 - PubMed
- Proc Natl Acad Sci U S A. 1981 Jan;78(1):459-63 - PubMed
- J Biol Chem. 1986 Feb 5;261(4):1782-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources