Genetic analysis of glucose regulation in saccharomyces cerevisiae: control of transcription versus mRNA turnover - PubMed (original) (raw)
Comparative Study
. 1996 Jan 15;15(2):363-74.
Affiliations
- PMID: 8617211
- PMCID: PMC449951
Comparative Study
Genetic analysis of glucose regulation in saccharomyces cerevisiae: control of transcription versus mRNA turnover
G P Cereghino et al. EMBO J. 1996.
Abstract
A major determinant of the steady-state level of the mRNA encoding the iron protein (Ip) subunit of succinate dehydrogenase of yeast is its rate of turnover. This mRNA is significantly more stable in glycerol than in glucose media. Many other genes, for example, SUC2, that are repressed in the presence of glucose are believed to be controlled at the level of transcription. The present study elucidates differences in the regulatory mechanisms by which glucose controls the transcription and turnover of the SUC2 and Ip mRNAs. The signaling pathway for glucose repression at the transcriptional level has been associated with a number of gene products linking glucose uptake with nuclear events. We have investigated whether the same genes are involved in the control of Ip mRNA stability. Phosphorylation of glucose or fructose is critical in triggering the transcript's degradation, but any hexokinase will do. Of the other known genes examined, most, with the exception of REG1, are not involved in determining the differential stability of the Ip transcript. Finally, our results indicate that differential stability on different carbon sources also plays a role in determining the steady-state level of the SUC2 mRNA. Thus, glucose repression includes both transcriptional and post-transcriptional mechanisms.
Similar articles
- Glucose-dependent turnover of the mRNAs encoding succinate dehydrogenase peptides in Saccharomyces cerevisiae: sequence elements in the 5' untranslated region of the Ip mRNA play a dominant role.
Cereghino GP, Atencio DP, Saghbini M, Beiner J, Scheffler IE. Cereghino GP, et al. Mol Biol Cell. 1995 Sep;6(9):1125-43. doi: 10.1091/mbc.6.9.1125. Mol Biol Cell. 1995. PMID: 8534911 Free PMC article. - The role of the 5' untranslated region (UTR) in glucose-dependent mRNA decay.
de la Cruz BJ, Prieto S, Scheffler IE. de la Cruz BJ, et al. Yeast. 2002 Jul;19(10):887-902. doi: 10.1002/yea.884. Yeast. 2002. PMID: 12112242 - Control of mRNA turnover as a mechanism of glucose repression in Saccharomyces cerevisiae.
Scheffler IE, de la Cruz BJ, Prieto S. Scheffler IE, et al. Int J Biochem Cell Biol. 1998 Nov;30(11):1175-93. doi: 10.1016/s1357-2725(98)00086-7. Int J Biochem Cell Biol. 1998. PMID: 9839444 Review. - Inhibition of translation initiation following glucose depletion in yeast facilitates a rationalization of mRNA content.
Lui J, Campbell SG, Ashe MP. Lui J, et al. Biochem Soc Trans. 2010 Aug;38(4):1131-6. doi: 10.1042/BST0381131. Biochem Soc Trans. 2010. PMID: 20659017 Review.
Cited by
- mRNA decapping activators Pat1 and Dhh1 regulate transcript abundance and translation to tune cellular responses to nutrient availability.
Vijjamarri AK, Gupta N, Onu C, Niu X, Zhang F, Kumar R, Lin Z, Greenberg ML, Hinnebusch AG. Vijjamarri AK, et al. Nucleic Acids Res. 2023 Sep 22;51(17):9314-9336. doi: 10.1093/nar/gkad584. Nucleic Acids Res. 2023. PMID: 37439347 Free PMC article. - A ribonuclease III involved in virulence of Mucorales fungi has evolved to cut exclusively single-stranded RNA.
Cánovas-Márquez JT, Falk S, Nicolás FE, Padmanabhan S, Zapata-Pérez R, Sánchez-Ferrer Á, Navarro E, Garre V. Cánovas-Márquez JT, et al. Nucleic Acids Res. 2021 May 21;49(9):5294-5307. doi: 10.1093/nar/gkab238. Nucleic Acids Res. 2021. PMID: 33877360 Free PMC article. - The interplay between transcription and mRNA degradation in Saccharomyces cerevisiae.
Das S, Sarkar D, Das B. Das S, et al. Microb Cell. 2017 Jul 3;4(7):212-228. doi: 10.15698/mic2017.07.580. Microb Cell. 2017. PMID: 28706937 Free PMC article. Review. - Snf1-Dependent Transcription Confers Glucose-Induced Decay upon the mRNA Product.
Braun KA, Dombek KM, Young ET. Braun KA, et al. Mol Cell Biol. 2015 Dec 14;36(4):628-44. doi: 10.1128/MCB.00436-15. Print 2016 Feb 15. Mol Cell Biol. 2015. PMID: 26667037 Free PMC article. - Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals.
Gagnon J, Lavoie M, Catala M, Malenfant F, Elela SA. Gagnon J, et al. PLoS Genet. 2015 Feb 13;11(2):e1005000. doi: 10.1371/journal.pgen.1005000. eCollection 2015 Feb. PLoS Genet. 2015. PMID: 25680180 Free PMC article.
References
- Mol Microbiol. 1991 Jun;5(6):1301-7 - PubMed
- Mol Biol Cell. 1995 Sep;6(9):1125-43 - PubMed
- Mol Cell Biol. 1992 May;12(5):2302-14 - PubMed
- Mol Cell Biol. 1992 Jul;12(7):2941-8 - PubMed
- Biotechniques. 1992 Jul;13(1):18-20 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases