Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior - PubMed (original) (raw)

Comparative Study

. 1996 May;284(2):177-91.

doi: 10.1007/s004410050578.

Affiliations

• PMID: 8625385
• DOI: 10.1007/s004410050578

Comparative Study

Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior

V C Cordes et al. Cell Tissue Res. 1996 May.

Abstract

We have used antibodies against different proteins of the nuclear pore complex to identify cytoplasmic annulate lamellae by immunocytochemistry at the light- and electron-microscopic level in various lines of cultured mammalian cells. Although annulate lamellae (AL) are seldom observed in some cell lines, they occur in large numbers in other lineages, also with cell-type-specific size distributions. AL are especially abundant and prominent in African green monkey kidney epithelial cells of line RC37 and bovine mammary gland epithelial, cells of line BMGE. We have studied the distribution of AL in relation to other organelles by using double-label immunofluorescence microscopy and have demonstrated a significant relationship between AL and rough endoplasmic reticulum. We have further shown that AL are disassembled during prophase and reassembled at the end of mitosis, almost concomitantly with the disassembly and reassembly of the nuclear envelope. Cultured mammalian cells that are rich in AL can therefore be used as suitable models for studies of the biogenesis of these lamellae, which can conveniently be detected by immunofluorescence microscopy.

PubMed Disclaimer

Similar articles

• Immunocytochemistry of annulate lamellae: potential cell biological markers for studies of cell differentiation and pathology.
  Cordes VC, Gajewski A, Stumpp S, Krohne G. Cordes VC, et al. Differentiation. 1995 Apr;58(4):307-12. doi: 10.1046/j.1432-0436.1995.5840307.x. Differentiation. 1995. PMID: 7641981
• [Structural organization and possible functional role of annulate lamellae containing cytoplasmic pores].
  Morozova KN, Gubanova NV, Kiseleva EV. Morozova KN, et al. Tsitologiia. 2005;47(8):667-78. Tsitologiia. 2005. PMID: 16706209 Review. Russian.
• The annulate lamellae--from obscurity to spotlight.
  Kessel RG. Kessel RG. Electron Microsc Rev. 1989;2(2):257-348. doi: 10.1016/0892-0354(89)90003-8. Electron Microsc Rev. 1989. PMID: 2491346 Review.

Cited by

• Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex.
  Hase ME, Cordes VC. Hase ME, et al. Mol Biol Cell. 2003 May;14(5):1923-40. doi: 10.1091/mbc.e02-09-0620. Mol Biol Cell. 2003. PMID: 12802065 Free PMC article.
• Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket.
  Krull S, Thyberg J, Björkroth B, Rackwitz HR, Cordes VC. Krull S, et al. Mol Biol Cell. 2004 Sep;15(9):4261-77. doi: 10.1091/mbc.e04-03-0165. Epub 2004 Jun 30. Mol Biol Cell. 2004. PMID: 15229283 Free PMC article.
• Insertional mutation of the Drosophila nuclear lamin Dm0 gene results in defective nuclear envelopes, clustering of nuclear pore complexes, and accumulation of annulate lamellae.
  Lenz-Böhme B, Wismar J, Fuchs S, Reifegerste R, Buchner E, Betz H, Schmitt B. Lenz-Böhme B, et al. J Cell Biol. 1997 Jun 2;137(5):1001-16. doi: 10.1083/jcb.137.5.1001. J Cell Biol. 1997. PMID: 9166402 Free PMC article.
• Centrosome maturation requires YB-1 to regulate dynamic instability of microtubules for nucleus reassembly.
  Kawaguchi A, Asaka MN, Matsumoto K, Nagata K. Kawaguchi A, et al. Sci Rep. 2015 Mar 5;5:8768. doi: 10.1038/srep08768. Sci Rep. 2015. PMID: 25740062 Free PMC article.
• Molecular Characterization and Functional Analysis of Annulate Lamellae Pore Complexes in Nuclear Transport in Mammalian Cells.
  Raghunayakula S, Subramonian D, Dasso M, Kumar R, Zhang XD. Raghunayakula S, et al. PLoS One. 2015 Dec 7;10(12):e0144508. doi: 10.1371/journal.pone.0144508. eCollection 2015. PLoS One. 2015. PMID: 26642330 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer