Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes - PubMed (original) (raw)
Herpes simplex ICP27 mutant viruses exhibit reduced expression of specific DNA replication genes
S L Uprichard et al. J Virol. 1996 Mar.
Abstract
Herpes simplex virus type 1 mutants with certain lesions in the ICP27 gene show a 5- to 10-fold reduction in viral DNA synthesis. To determine how ICP27 promotes amplification of viral DNA, we examined the synthesis, accumulation, and stability of the essential viral replication proteins and steady-state levels of the replication gene transcripts throughout the course of ICP27 mutant virus infections. These studies reveal that in the absence of ICP27, expression of the UL5, UL8, UL52, UL9, UL42, and UL30 genes is significantly reduced at the level of mRNA accumulation. In contrast to that of these beta genes, ICP8 expression is unaltered in mutant virus-infected cells, indicating that ICP27 selectively stimulates only a subset of herpes simplex virus beta genes. Analysis of multiple ICP27 mutant viruses indicates a quantitative correlation between the ability of these mutants to replicate viral DNA and the level of replication proteins produced by each mutant. Therefore, we conclude that the primary defect responsible for restricted viral DNA synthesis in cells infected with ICP27 mutants is insufficient expression of most of the essential replication genes. Of further interest, this analysis also provides new information about the structure of the UL52 gene transcripts.
Similar articles
- Aberrant RNA polymerase initiation and processivity on the genome of a herpes simplex virus 1 mutant lacking ICP27.
Birkenheuer CH, Baines JD. Birkenheuer CH, et al. J Virol. 2024 Jun 13;98(6):e0071224. doi: 10.1128/jvi.00712-24. Epub 2024 May 23. J Virol. 2024. PMID: 38780246 - Functional order of assembly of herpes simplex virus DNA replication proteins into prereplicative site structures.
Liptak LM, Uprichard SL, Knipe DM. Liptak LM, et al. J Virol. 1996 Mar;70(3):1759-67. doi: 10.1128/JVI.70.3.1759-1767.1996. J Virol. 1996. PMID: 8627698 Free PMC article. - HSV-1 DNA Replication-Coordinated Regulation by Viral and Cellular Factors.
Packard JE, Dembowski JA. Packard JE, et al. Viruses. 2021 Oct 7;13(10):2015. doi: 10.3390/v13102015. Viruses. 2021. PMID: 34696446 Free PMC article. Review. - The two helicases of herpes simplex virus type 1 (HSV-1).
Chattopadhyay S, Chen Y, Weller SK. Chattopadhyay S, et al. Front Biosci. 2006 Sep 1;11:2213-23. doi: 10.2741/1964. Front Biosci. 2006. PMID: 16720308 Review.
Cited by
- Suppression of HSV-1 infection and viral reactivation by CRISPR-Cas9 gene editing in 2D and 3D culture models.
Bellizzi A, Çakır S, Donadoni M, Sariyer R, Liao S, Liu H, Ruan GX, Gordon J, Khalili K, Sariyer IK. Bellizzi A, et al. Mol Ther Nucleic Acids. 2024 Jul 19;35(3):102282. doi: 10.1016/j.omtn.2024.102282. eCollection 2024 Sep 10. Mol Ther Nucleic Acids. 2024. PMID: 39176174 Free PMC article. - Herpes Simplex Virus-1 ICP27 Nuclear Export Signal Mutants Exhibit Cell Type-Dependent Deficits in Replication and ICP4 Expression.
Sanders LS 3rd, Comar CE, Srinivas KP, Lalli J, Salnikov M, Lengyel J, Southern P, Mohr I, Wilson AC, Rice SA. Sanders LS 3rd, et al. J Virol. 2023 Jul 27;97(7):e0195722. doi: 10.1128/jvi.01957-22. Epub 2023 Jun 13. J Virol. 2023. PMID: 37310267 Free PMC article. - The Role of Pyrazolopyridine Derivatives on Different Steps of Herpes Simplex Virus Type-1 In Vitro Replicative Cycle.
Miranda MD, Chaves OA, Rosa AS, Azevedo AR, Pinheiro LCDS, Soares VC, Dias SSG, Abrantes JL, Bernardino AMR, Paixão ICP, Souza TML, Fontes CFL. Miranda MD, et al. Int J Mol Sci. 2022 Jul 23;23(15):8135. doi: 10.3390/ijms23158135. Int J Mol Sci. 2022. PMID: 35897709 Free PMC article. - Antiviral mechanism of carvacrol on HSV-2 infectivity through inhibition of RIP3-mediated programmed cell necrosis pathway and ubiquitin-proteasome system in BSC-1 cells.
Wang L, Wang D, Wu X, Xu R, Li Y. Wang L, et al. BMC Infect Dis. 2020 Nov 11;20(1):832. doi: 10.1186/s12879-020-05556-9. BMC Infect Dis. 2020. PMID: 33176697 Free PMC article. - Antiviral activity of mitoxantrone dihydrochloride against human herpes simplex virus mediated by suppression of the viral immediate early genes.
Huang Q, Hou J, Yang P, Yan J, Yu X, Zhuo Y, He S, Xu F. Huang Q, et al. BMC Microbiol. 2019 Dec 7;19(1):274. doi: 10.1186/s12866-019-1639-8. BMC Microbiol. 2019. PMID: 31812160 Free PMC article.
References
- J Virol. 1994 Jul;68(7):4251-61 - PubMed
- J Biol Chem. 1994 Nov 18;269(46):29329-34 - PubMed
- J Virol. 1984 Oct;52(1):108-18 - PubMed
- J Virol. 1975 Aug;16(2):275-83 - PubMed
- J Virol. 1994 Aug;68(8):4797-810 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources