DNA: an extensible molecule - PubMed (original) (raw)
DNA: an extensible molecule
P Cluzel et al. Science. 1996.
Abstract
The force-displacement response of a single duplex DNA molecule was measured. The force saturates at a plateau around 70 piconewtons, which ends when the DNA has been stretched about 1.7 times its contour length. This behavior reveals a highly cooperative transition to a state here termed S-DNA. Addition of an intercalator suppresses this transition. Molecular modeling of the process also yields a force plateau and suggests a structure for the extended form. These results may shed light on biological processes involving DNA extension and open the route for mechanical studies on individual molecules in a previously unexplored range.
Similar articles
- Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules.
Smith SB, Cui Y, Bustamante C. Smith SB, et al. Science. 1996 Feb 9;271(5250):795-9. doi: 10.1126/science.271.5250.795. Science. 1996. PMID: 8628994 - Secondary structure of double-stranded DNA under stretching: elucidation of the stretched form.
Maaloum M, Beker AF, Muller P. Maaloum M, et al. Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Mar;83(3 Pt 1):031903. doi: 10.1103/PhysRevE.83.031903. Epub 2011 Mar 7. Phys Rev E Stat Nonlin Soft Matter Phys. 2011. PMID: 21517521 - B-S transition in short oligonucleotides.
Morfill J, Kühner F, Blank K, Lugmaier RA, Sedlmair J, Gaub HE. Morfill J, et al. Biophys J. 2007 Oct 1;93(7):2400-9. doi: 10.1529/biophysj.107.106112. Epub 2007 Jun 8. Biophys J. 2007. PMID: 17557787 Free PMC article. - Denaturation transition of stretched DNA.
Hanke A. Hanke A. Biochem Soc Trans. 2013 Apr;41(2):639-45. doi: 10.1042/BST20120298. Biochem Soc Trans. 2013. PMID: 23514169 Review. - A Tour de Force on the Double Helix: Exploiting DNA Mechanics To Study DNA-Based Molecular Machines.
Wasserman MR, Liu S. Wasserman MR, et al. Biochemistry. 2019 Nov 26;58(47):4667-4676. doi: 10.1021/acs.biochem.9b00346. Epub 2019 Jun 28. Biochemistry. 2019. PMID: 31251042 Free PMC article. Review.
Cited by
- Stretching and bending fluctuations of short DNA molecules.
Padinhateeri R, Menon GI. Padinhateeri R, et al. Biophys J. 2013 Jan 22;104(2):463-71. doi: 10.1016/j.bpj.2012.11.3820. Biophys J. 2013. PMID: 23442868 Free PMC article. - Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.
Dorfman KD, King SB, Olson DW, Thomas JD, Tree DR. Dorfman KD, et al. Chem Rev. 2013 Apr 10;113(4):2584-667. doi: 10.1021/cr3002142. Epub 2012 Nov 12. Chem Rev. 2013. PMID: 23140825 Free PMC article. Review. No abstract available. - Magnetic tweezers: micromanipulation and force measurement at the molecular level.
Gosse C, Croquette V. Gosse C, et al. Biophys J. 2002 Jun;82(6):3314-29. doi: 10.1016/S0006-3495(02)75672-5. Biophys J. 2002. PMID: 12023254 Free PMC article. - Dual binding modes for an HMG domain from human HMGB2 on DNA.
McCauley M, Hardwidge PR, Maher LJ 3rd, Williams MC. McCauley M, et al. Biophys J. 2005 Jul;89(1):353-64. doi: 10.1529/biophysj.104.052068. Epub 2005 Apr 15. Biophys J. 2005. PMID: 15833996 Free PMC article. - Micromechanical studies of mitotic chromosomes.
Poirier MG, Marko JF. Poirier MG, et al. J Muscle Res Cell Motil. 2002;23(5-6):409-31. J Muscle Res Cell Motil. 2002. PMID: 12785094 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources